乙酰胆碱和血小板激活因子含量变化与血管性痴呆发生机制的相关性

摘要

目的动态观察乙酰胆碱和血小板激活因子含量变化对血管性痴呆( vascular dementia, VD)发生机制的意义.方法 Wistar 大鼠共 30只 ,将大鼠随机分为模型组 (A组 )和对照组 ( B组 ).模型组用 "0"号丝线间歇阻断双侧颈总动脉 10 min,共阻断双侧颈总动脉 3次 ,每次间隔 10 min,同时尾静脉放血 2 ml,对照组仅分离双侧颈总动脉.用比色法和高效薄层色谱法,分别测定血管性痴呆大鼠手术后,第 2,3,5天海马乙酰胆碱( acetylcholine, ACh)和血小板激活因子 (platelet activating factor, PAF)含量.结果手术后第 2,3,5天大鼠海马 ACh含量,在模型组与对照组间差异均有显著性意义 (F=20.53,P < 0.05);而 PAF含量两组间则差异均无显著性意义 (F=2.98,P >0.05).结论血管性痴呆大鼠海马 ACh 含量的持续降低,可能是 VD发生.发展的重要原因之一 ;而 PAF则可能主要参与急性脑缺血早期的病理生理过程,对脑缺血后期的病理生理变化意义不大.%Aim To observe the significance of dynamic changes of acetylcholine and platelet activating factor in pathogenic mechanism of vascular dementia. Methods 30 Wistar rats were randomly divided into model group (group A) and control group(group B). In model grouop,bilateral common carotid arteries were intermittently blocked with silk suture of 0 size for 10 minutes ,reperfused for 10 minutes.Common carotid artery was blocked for 3 times in total with 10 minutes of intervals.At the same time,2 ml of blood was withdrawn.In control group,bilateral common carotid arteries were only separated. Concentration of acetylcholie(Ach) and platelet activating factor(PAF) in rats, hippocampuses were measured with high performance thin- layer chromatography and colorimetric method.Results 2,3,5 day after operation , there was significant difference in the concentration of ACch(F=20.530,P< 0.05), no significant difference in PAF between the two groups(F=2.98,P >0.05). Conclusion The continuous decline of ACh concentration of hippocampuses may be one of the reasons for VaD occurrence and development; but PAF may participate mainly in the pathophysiological course in the early stage and have few effect in the later stage of cerebral ischemia.