脐带间充质干细胞联合C5a受体拮抗剂移植治疗脑损伤大鼠的功能恢复

摘要

BACKGROUND: C5a is an important mediator and chemokine of inflammation .which plays an important role in the process oftraumatic brain injury (TBI) pathogenesis.OBJECTIVE: To investigate the effect of human umbilical cord mesenchymalstem cells (hU C-MSCs)transplantation andC5areceptor antagonist on TBI.METHODS: Sprague-Dawley rats were prepared to the heavy-duty hydraulic traumatic brain injury models and they wererandomly divided into three groups: TBI group. hUC-MSCs transplantation group and hUC-MSCs transplantation plus C5areceptor antagonist group(C5a+hUC-MSCs group).RESULTS AND CONCLUSION: At 1-4 weeks after transplantation, the modified neurologicals everity scores (mNSS)were lowerin the hUC-MSCs and Csa+hUC-MSCs groups than in the TBI group (p < 0.05.001). While mNSS were decreased significantly inthe C5a+hUC-MSCs group. The average time of escape latency was gradually decreased in each group; however, it was muchshorter in the C5a+hUC-MSCs group than in the other two groups at 3-5 days (p < 0.05). The frequency of platform passing andthe percentage of swimming distance traveled in the previous target quadrant in the C5a+hUC-MSCs group were significantlygreater than those in the TBI group and hUC-MSCs group. It is indicated that UC-MSCs transplantation combined with C5areceptor antagonist can significantly improve the neurological function in the rats.%背景:补体系统活化产物过敏毒素 C5a是炎症反应的重要递质和趋化因子,在创伤性脑损伤病理发生过程中起重要作用.目的:脐带间充质干细胞移植同时应用C5a受体拮抗剂,观察两者对脑损伤大鼠功能恢复的影响.方法:SD大鼠制备重型液压颅脑损伤模型,随机数字表均分成为脑损伤组、脐带间充质干细胞组和C5a受体拮抗剂+脐带间充质干细胞组.结果与结论:移植后1~4周,与脑损伤组比较,脐带间充质干细胞和C5a受体拮抗剂+脐带间充质干细胞组神经学缺损评分明显降低(P < 0.05,0.01),后者降低更显著.各组平均潜伏时间均逐渐缩短,C5a受体拮抗剂+脐带间充质干细胞组3~ 5 d时最短(P < 0.05),穿越平台次数及在目标象限游泳距离与总距离百分比均高于其他两组(P < 0.05).提示脐带间充质干细胞移植可明显改善重型颅脑损伤后大鼠的神经学功能,联合应用C5a受体拮抗剂有协同效果.