纳米TiO2：C薄膜涂层的生物相容性评价**☆◆

摘要

BACKGROUND: In previous experiments, TiO2:C thin film coating have been prepared on the surface of titanium paper by sol-gel process. OBJECTIVE: To evaluate the biocompatibility of nanometer TiO2:C thin film coating. METHODS: Hemolysis test: New Zealand white rabbit venous blood was extracted and added into the TiO2-normal saline, TiO2:C-normal saline, distil ed water and fresh normal saline. Cytotoxicity test: TiO2:C leaching liquor was added into the L929 cel culture medium in the logarithmic growth phase. Short-term systemic toxicity test: 60 BALB/C mice were randomly divided into three groups and lavaged with leaching liquor extracted from TiO2, TiO2:C coatings and normal saline. Oral mucosa stimulation test: the gutta-percha wafer and the nano-TiO2:C thin film coating were sutured and fixed to the cheek pouch of inaurate hamster. RESULTS AND CONCLUSION: Acute hemolysis was not induced by nano-TiO2:C thin film coating. Toxicity level of nano-TiO2:C thin film coating was 0-1. It had no obvious toxicity to the cells. There was no obvious abnormality of cel cycle. TiO2:C leaching liquor had no short-term systemic toxicity on mice and no stimulation to oral mucosa of hamster. The results showed that nano-TiO2:C thin film coatings have good bio-security.%　　背景：前期实验采用溶胶-凝胶法在纯钛片表面制备了纳米 TiO2：C 薄膜涂层。目的：评估纳米 TiO2：C 薄膜涂层的生物相容性。方法：①溶血实验：取新西兰大白兔静脉血，分别加到 TiO2-生理盐水、TiO2：C-生理盐水、蒸馏水和生理盐水中。②细胞毒性实验：将 TiO2：C 浸提液加入对数生长期的 L929细胞培养基中。③短期全身毒性实验：将60只 BALB/C 小白鼠随机分为3组，分别灌胃给予 TiO2浸提液、TiO2：C 浸提液及生理盐水。④口腔黏膜刺激实验：将牙胶圆片与 TiO2：C 纳米薄膜涂片分别缝合固定于仓地鼠颊黏膜上。结果与结论：纳米 TiO2：C 涂层未引起急性溶血，毒性等级为0-1级，未对细胞产生明显毒性，细胞周期未见明显异常；TiO2：C 浸提液对小鼠无短期全身毒性；对仓鼠口腔黏膜无刺激性。表明纳米 TiO2：C薄膜涂层具有良好的生物安全性。