BACKGROUND:FoxO1 participates in diverse processes of cellphysiology, including cellproliferation and apoptosis, reactive oxygen species, longevity, cancer, the regulation of cellcycle and metabolism. Vibration loading is believed to promote metabolism, delay muscle fatigue, and facilitate adaptive bone reconstruction. OBJECTIVE:To investigate the effects of whole-body vertical vibration on the protein expression of FoxO1 in tibia and bone marrow cellof ovariectomized (OVX) osteoporosis rats. METHODS:Thirty-six healthy 3-month-old female Sprague-Dawley rats were randomly divided into the fol owing three groups by body weight:sham-operation (sham) group, OVX group, and OVX whole-body vertical vibration (OVX+V) group. At the 11th week after operation, the OVX+V group rats were vibrated on a vibration platform twice per day for 7 weeks according to the fol owing schedule:whole-body vertical vibration for 15 minutes, with an interval of 10 minutes. The frequency was 90 Hz and the amplitude was 0.5 mm. Within 24-48 hours after the last treatment, the fifth lumbar vertebra as wel as the right femur and tibia were isolated. The bone mineral density of the fifth lumbar vertebra and right femur was detected by dual-energyΧ-ray absorptiometry. The FoxO1 protein expression in proximal tibia and bone marrow cells was detected by western blot assay. RESULTS AND CONCLUSION:As revealed by the results of bone mineral density, the bone mineral densities of the proximal and distal femur as wel as the fifth lumbar vertebra in OVX+V group were significantly increased while no significant difference could be seen in the mid shaft. As revealed by the results of western blot, there was no FoxO1 protein expression in the tibia, but it could be detected in the bone marrow cells. Compared with the sham group, there was no significant difference in FoxO1 protein expression in bone marrow cells of OVX group;compared with the OVX group, the protein expression level of FoxO1 in bone marrow cells of the OVX+V group was significantly reduced. The results suggested that whole-body vertical vibration could inhibit the protein expression of FoxO1 in bone marrow cells of osteoporosis rats.%背景：FoxO1蛋白参与多种多样的细胞和生理过程，包括细胞增殖与凋亡、活性氧的反应、长寿、癌症、细胞周期和代谢的调节等。研究认为振动负荷能促进新陈代谢，延缓肌肉疲劳，加速骨适应性重建。 目的：进一步验证全身垂直振动对去卵巢骨质疏松大鼠骨组织和骨髓细胞叉头框转录因子 O 亚族1(FoxO1)蛋白表达的影响。 方法：将36只健康3月龄雌性SD大鼠，按体质量分层后随机分为假手术组、去卵巢静止组和去卵巢振动组。在手术后第11周，对去卵巢振动组大鼠实施7周每天不间断的全身垂直振动治疗，2次/d，每次振动训练15 min，间隔10 min，振动频率为90 Hz，振动幅度为0.5 mm。于末次处理结束24-48 h内，游离各组大鼠第5腰椎、右侧股骨和胫骨，用双能X射线骨密度仪检测右股骨和第5腰椎的离体骨密度，用Western blot检测右胫骨近端和骨髓细胞FoxO1蛋白的表达。 结果与结论：骨密度检测结果显示，与去卵巢静止组比较，去卵巢振动组大鼠股骨远端和近端以及第5腰椎骨密度显著增加，而股骨中段无显著变化。Western blot检测结果显示：FoxO1蛋白在胫骨组织中不表达，但在骨髓细胞中表达。与假手术组比较，去卵巢静止组大鼠骨髓细胞FoxO1蛋白表达水平无显著变化；与去卵巢静止组比较，去卵巢振动组大鼠骨髓细胞FoxO1蛋白表达水平显著下降。结果表明，全身垂直振动能抑制FoxO1蛋白在去卵巢大鼠骨髓细胞中的表达。
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