正畸力作用下大鼠炎性牙周组织中肝细胞生长因子的表达

摘要

BACKGROUND:The cytokine has an effect of immunoregulation and immediate induction in the reconstruction of periodontal tissue. At present, the role and mechanism of hepatocyte growth factor involving the reconstruction of periodontal tissue under orthodontic force are stil unclear. OBJECTIVE:To explore the mechanism underlying hepatocyte growth factor in the tooth movement and periodontal tissues remodeling under the inflammation periodontal tissue condition. METHODS:Thirty Sprague-Dawley rats, aged 8 weeks, were used to establish periodontitis model. The obtained model was randomly divided into two groups:inflammatory control group and inflammatory force group. In the force group, rats were treated with the fixed orthodontic appliance by 50 g forces in the maxil ary first molars. After 1, 3, 5, 7 and 14 days of tooth movement, five rats were sacrificed respectively. Hematoxylin-eosin staining and immunohistochemistry methods were used to analyze the expression and distribution of hepatocyte growth factor in the periodontium for rats at different tooth movement stages. RESULTS AND CONCLUSION:Hematoxylin-eosin staining results showed that, remodeling of periodontal tissues existed in al groups. (2) The immunohistochemical results showed that hepatocyte growth factor had positive expression in periodontal tissue, and the distribution was even in the control group. In the force group, hepatocyte growth factor expression was increased and reached the peak on day 5, then began to decline. Osteoblast, fibroblast and osteoclast were strongly expressed. The findings indicate that, hepatocyte growth factor is involved in the periodontal tissues remodeling under orthodontic force, and inflammation can increase the expression of hepatocyte growth factor in periodontal tissue.%背景：细胞因子在牙周组织改建机制中具有重要的免疫调节和直接介导作用。目前肝细胞生长因子在正畸力作用后对牙周组织改建的作用及机制尚未见报道。　　目的：探讨肝细胞生长因子在炎性牙周条件下参与牙移动及牙周改建的机制。　　方法：选用30只8周龄雄性SD大鼠，建立牙周炎模型，随机分为2组，炎性对照组5只，炎性加力组25只。炎性加力组在上颌第一磨牙近中施加50 g的力值，分别在受力1，3，5，7，14 d处死，每次处死5只。采用苏木精-伊红染色、免疫组化方法分析牙齿受力移动不同阶段牙周组织中肝细胞生长因子表达和分布变化。　　结果与结论：苏木精-伊红染色结果显示，受力牙齿与未受力牙齿牙周组织中都存在一定程度的改建。免疫组化结果显示，炎性对照组中可观察到肝细胞生长因子在牙周组织中呈阳性表达，分布较均匀。炎症加力组大鼠牙周组织内肝细胞生长因子受力后表达增高，在第5天达到高峰，之后开始下降；其中成骨细胞、成纤维细胞及破骨细胞均为强表达。提示肝细胞生长因子参与了正畸牙周组织的改建过程，其表达随时间呈现一定规律，炎症刺激可导致牙周组织中肝细胞生长因子的表达增高。