背景：新型带负电荷硫酸钙/β-磷酸三钙复合骨水泥具有成骨效应，但目前尚无其应用于椎体成形的相关研究。 目的：通过动物实验评估新型带负电荷硫酸钙/β-磷酸三钙复合骨水泥在体内的生物力学性质及成骨性能。 方法：取30只新西兰大白兔建立骨质疏松模型4周后，在每只兔L 3、L 5椎体中央制造骨缺损，分别注射新型带负电荷硫酸钙/β-磷酸三钙复合骨水泥(实验组)与聚甲基丙烯酸甲酯骨水泥(对照组)，L 1椎体为未干预的骨质疏松模型。于术后3，6个月各处死15只，取3组椎体进行Micro-CT分析及生物力学实验。 结果与结论：①MicroCT三维重建显示，术后3个月时，实验组骨小梁三维结构参数明显优于对照组(P<0.05)，与骨质疏松模型组无显著差别(P>0.05)；术后6个月时，实验组骨小梁三维结构参数明显优于对照组和骨质疏松模型组(P均<0.05)。②术后3个月时，实验组椎体强度明显小于对照组(P <0.05)，但高于骨质疏松模型组(P <0.05)；椎体刚度小于对照组和骨质疏松模型组(P均<0.05)。术后6个月时，实验组椎体强度与对照组接近(P >0.05)，但高于骨质疏松模型组(P <0.05)；椎体刚度与对照组和骨质疏松模型组无差异(P >0.05)。表明新型带负电荷硫酸钙/β-磷酸三钙复合骨水泥可以迅速有效地提高椎体生物力学强度，具有诱导成骨效应。%BACKGROUND:A newly developed calcium phosphate/β-tricalcium phosphate composite bone cement with a negative surface charge (genex®) has been reported to possess osteoinductivity properties. However, to our knowledge, no previous literatures have reported genex® for vertebroplasty in the osteoporotic spine. OBJECTIVE:To evaluate the biomechanical properties and osteogenesis of vertebral bodies injected with genex® cement in a rabbit vertebroplasty defect model. METHODS:Thirty New Zealand rabbits were used to establish osteoporosis models. Four weeks after modeling, model rabbits had an iatrogenical y created cavitary lesion at L 3 and L 5 and were injected with either genex® cement (experimental group) or polymethyl methacrylate bone cement (control group). The L 1 vertebral body served as model group without treatment. After 3 and 6 months, 15 rats from each group were executed respectively, and three vertebral samples were taken for Micro-CT analysis and biomechanical tests. RESULTS AND CONCLUSION:(1) The Micro-CT showed better three-dimensional structure parameters of the trabecular bone in the experimental group than the control group (P<0.05) after 3 months, which however had no difference from the model group (P>0.05). After 6 months, the structure parameters in the experimental group were superior to those in the control and model groups (P<0.05). (2) After 3 months, the vertebral body compression strength of the experimental group was lower than that of the control group (P<0.05), but higher than that in the model group (P<0.05). The vertebral stiffness of the experimental group was lower than that in control and model groups (P<0.05). After 6 months, the vertebral body compression strength of the experimental group was not different from that of the control group (P>0.05), but stil higher than that of the model group (P<0.05). The vertebral stiffness showed no difference between three groups (P>0.05). These findings indicate that genex® cement can rapidly repair osteoporotic vertebral defects and improve the bone strength. Verterbroplasty with genex® cement has adequate osteoinductivity, biocompatibility, and adequate compressive strength.
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