铁铬钼软磁合金表面镀铬后的生物相容性

摘要

背景：研究表明，铁铬钼系列软磁合金具有理想的磁性能和机械加工性能，表面镀 Cr6+处理可明显增强其在口腔环境中的耐腐蚀性，但其表面镀铬后的生物安全性仍需进一步检验。目的：评价铁铬钼软磁合金表面镀铬后的生物相容性。方法：取对数生长期L-929细胞悬液，以6×107 L-1的细胞浓度接种于96孔板中，分别加入纯钛浸提液、铁铬钼软磁合金原样浸提液、铁铬钼电镀Cr6+软磁合金浸提液与聚氯乙烯浸提液培养。培养5 d后，观察细胞形态和贴壁情况，采用CCK-8法检测细胞A值，并计算各组细胞相对增殖率，评价材料细胞毒性分级。结果与结论：纯钛浸提液组细胞形态正常，贴壁生长良好，表现为无细胞毒性；铁铬钼软磁合金原样浸提液组细胞形态和生长状态均良好，偶见个别细胞溶解，培养液内出现散在红褐色颗粒，表现为无或极轻微的细胞毒性；铁铬钼镀 Cr6+软磁合金浸提液组细胞生长状态良好，表现为无或极轻微的细胞毒性；聚氯乙烯浸提液组超过70%细胞固缩或溶解呈空泡状，大量细胞碎片，超过50%细胞生长抑制，表现中度以上的细胞毒性。铁铬钼电镀Cr6+软磁合金浸提液的细胞毒性为0至1级。表明镀铬的铁铬钼软磁合金具有良好的生物相容性。%BACKGROUND:Studies have demonstrated that Fe-Cr-Mo soft magnetic aloy has desired magnetic properties and machining properties. Surface Cr6+ plating treatment can significantly enhance the corrosion resistance in oral environment, but its biosecurity needs further testing. OBJECTIVE: To evaluate the biocompatibility of Fe-Cr-Mo soft magnetic aloy with chromium plating. METHODS:Logarithmic growth phase L929 cel suspension was obtained and seeded in 96-wel plates at a cel concentration of 6×107/L. Pure titanium extract, Fe-Cr-Mo soft magnetic aloy original extract, Cr6+-plated Fe-Cr-Mo soft magnetic aloy and PVC extract were added respectively. After 5 days of culture, cel morphology and adherent circumstances were observed. The absorbance value was detected using cel counting kit-8 assay. The relative growth rate of cels in each group was calculated. The cytotoxicity grades of materials were evaluated. RESULTS AND CONCLUSION:In the pure titanium extracts group, the cels with normal morphology showed good adherent growth and no cytotoxicity. In the Fe-Cr-Mo soft magnetic aloy original extract group, the cel morphology and growth status were both good, with occasionaly individual cel lysis. Scattered reddish brown particles were visible in the culture solution, showing no or very mild cytotoxicity. In Cr6+-plated Fe-Cr-Mo soft magnetic aloy extract group, the cels grew wel and showed no or very mild cytotoxicity. In PVC extract group, more than 70% of cels were vacuole-shaped and presented with pyknosis or dissolution, there was a large number of cel debris, and cel growth was inhibited in over 50% cels, showing moderate cytotoxicity or above. The cytotoxicity of Cr6+-plated Fe-Cr-Mo soft magnetic aloy extract was grade 0-1. These results demonstrate that Fe-Cr-Mo soft magnetic aloywith chromium plating has good biocompatibility.