5-氟尿嘧啶对人舌鳞癌Tca8113中肿瘤干细胞的富集作用

摘要

背景：肿瘤对于化疗药物具有耐药性，而耐药性产生又与肿瘤干细胞密切相关。因此，如何靶向杀伤肿瘤干细胞，将成为口腔鳞癌治疗的关键。目的：探讨5-氟尿嘧啶对舌鳞癌Tca8113肿瘤细胞生物学特性的影响。方法：利用CCK-8实验检测不同质量浓度5-氟尿嘧啶对Tca8113细胞活性的影响，筛选相对较好的药物质量浓度和作用时间进行后续实验，以不添加5-氟尿嘧啶为对照组，流式细胞仪检测细胞周期及肿瘤细胞中侧群细胞比例的变化，划痕实验检测肿瘤细胞迁移能力变化。结果与结论：CCK-8检测显示：5-氟尿嘧啶对舌鳞癌Tca8113细胞系细胞的活性抑制呈时间及药物浓度的正相关关系，50 mg/L 5-氟尿嘧啶作用48 h时，细胞活性抑制效果相对较好。流式细胞仪细胞周期检测显示：实验组G0/G1期细胞比例较对照组明显升高(P=0.01)，S期细胞较对照组下降(P=0.244)，实验组G2/M期细胞完全消失(P=0.00)。5-氟尿嘧啶处理后侧群细胞比例较处理前明显升高(P=0.00)。细胞划痕实验显示实验组划痕愈合能力较对照组增强。结果表明5-氟尿嘧啶药物筛选人舌鳞癌Tca8113细胞可以作为富集类肿瘤干细胞的方法之一。%BACKGROUND:Tumor cels are resistant to chemotherapeutic drugs, and drug resistance is closely correlated with tumor stem cels. Therefore, how to kil tumor stem cels wil become the key to the treatment of oral squamous cel carcinoma. OBJECTIVE:To study the effect of 5-fluorouracil on biological characteristics of tongue squamous cel carcinoma Tca8113 cels. METHODS:Viability of Tca8113 cels treated with different concentrations of 5-fluorouracil was determined by cel counting kit-8, and the best drug concentration and time were screened for subsequent experiments. Tca8113 cels without 5-fluorouracil acted as control group. Then the cel cycle and percentage of the side population cels in Tca8113 cels were determined by flow cytometry. Scratch test was used to determine the migration ability of Tca8113 cels. RESULTS AND CONCLUSION:Results from cel counting kit-8 showed that 5-fluorouracil inhibited the viability of Tca8113 cels positively in a time- and dose-dependent manner. Tca8113 cels under intervention with 50 mg/L 5-fluorouracil for 48 hours showed lowest cel viability. Flow cytometry results showed that in the experimental group, G0/G1 phase cels increased significantly compared with the control group (P=0.01), S phase cels decreased significantly compared with the control group (P=0.244), and G2/M phase cels disappeared completely. After treatment with 5-fluorouracil, the percentage of side population cels was increased significantly (P=0.00). The scratch test showed that in the experimental group, the cels had better ability of wound healing than those in the control group. In conclusion, 5-fluorouracil can enrich the cancer stem cel population in Tca8113 cels.