树鼩血清与多种二抗的交叉反应:可在树鼩疾病动物模型中广泛应用

摘要

背景：由于树鼩是介于食虫目和灵长目之间的代表，进化程度高，较价廉的灵长类动物，医学生物学的用途很高，已受到广大学者的重视。n　　目的：检测通常用的二抗能否与树鼩的血清发生免疫反应。n　　方法：用Western和ELISA的方法检测树鼩的血清是否与抗兔、抗羊、抗人、抗小鼠、抗大鼠、抗猴的二抗发生交叉反应。n　　结果与结论：Western 结果表明树鼩的血清与抗兔、抗羊、抗人、抗小鼠、抗大鼠的二抗均不发生反应，与抗猴的二抗有交叉反应。ELISA 的结果也表明树鼩的血清与抗猴的二抗有交叉反应，而与其他二抗没有交叉反应。结果说明通常卖的二抗不能用于树鼩IgG的免疫检测，只有抗猴的二抗与树鼩的血清发生交叉反应，必需制备抗树鼩IgG的单克隆和多克隆抗体，在没有现成抗体可用时，可采用抗猴的二抗替代检测，能在树鼩疾病动物模型的研究中得到广泛应用。%BACKGROUND:Tree shrew is a representative between insectivore and primates, has a high degree of evolution, is more inexpensive primates, has high use of medical biology, and has been attached by scholars. OBJECTIVE:To detect whether the commonly used secondary antibodies have immune response with tree shrew serum. METHODS:Western blot assay and enzyme linked immunosorbent assay were utilized to detect whether the tree shrew serum had cross-reacts with anti-rabbit, anti-goat, anti-human, anti-mouse, anti-rat, and anti-monkey secondary antibodies. RESULTS AND CONCLUSION:Western blot assay results indicated that tree shrew serums did not react with anti-rabbit, anti-goat, anti-human, anti-mouse, and anti-rat secondary antibodies and had cross reaction with anti-monkey secondary antibody. Enzyme linked immunosorbent assay results also indicated that tree shrew serums were cross-reactive with anti-monkey secondary antibody, but did not have cross-reactivity with the other secondary antibodies. Above data confirmed that the usual y soled secondary antibody cannot be used to immunoassay with tree shrews IgG. Only anti-monkey secondary antibody has cross-react with tree shrew serum. It is necessary to prepare anti-tree shrew IgG monoclonal and polyclonal antibodies. When no antibody is readily available at present, anti-monkey secondary antibody can be used to substitute detection, and can be widely applied in the study of tree shrew models of disease.