壳聚糖与海藻酸钙双相陶瓷骨支架的机械性能及细胞相容性

摘要

BACKGROUND: Hydroxyapatite/β-tricalcium phosphate biphasic ceramic bone has good cel compatibility, but its mechanical properties are poor. OBJECTIVE: To construct chitosan/ or calcium alginate/biphasic ceramic bone scaffolds and to detect their mechanical properties and cytocompatibility. METHODS: Different concentrations of chitosan (2%, 4%, 7%, 10%) or calcium alginate (3%, 4%, 5%, 7%) were mixed with biphasic ceramic bone to prepare chitosan/biphasic ceramic bone scaffold and calcium alginate/biphasic ceramic bone scaffold. Their morphology and structure, coagulation time, anti-dissolution properties, shear force, compressive strength and cel compatibility were detected. RESULTS AND CONCLUSION: (1) Coagulation time: with the concentration increase, the initial and final setting time of these two kinds of composite scaffolds were prolonged to some extent. (2) Scanning electron microscopy: these two kinds of composite scaffolds showed porous microstructures with different pore sizes. (3) Anti-dissolution properties: the calcium alginate/biphasic ceramic bone scaffold (3%, 4%, 5%, 7%) and chitosan/biphasic ceramic bone scaffold (7%, 10%) had good anti-dissolution properties in the liquid. (4) Mechanical strength: with the concentration increase, the shear force and compressive strength of the calcium alginate/biphasic ceramic bone scaffold were reduced. (5) Cel compatibility: the cytotoxicity of chitosan/ or calcium alginate/biphasic ceramic bone scaffolds was graded as 0-1 or 2-3, respectively. These results show that the chitosan/biphasic ceramic bone scaffold has better mechanical properties and cel compatibility than the calcium alginate/biphasic ceramic bone scaffold.%背景：由羟基磷灰石和少量β-磷酸三钙构成的双相陶瓷骨具有良好的细胞相容性，但机械性能较差。目的：构建壳聚糖、海藻酸钠胶体与双相陶瓷骨复合支架材料，检测其机械性能和细胞相容性。方法：将不同浓度的壳聚糖(2%、4%、7%、10%)、海藻酸钠胶体(3%、4%、5%、7%)分别与双相陶瓷骨混合，制备壳聚糖/双相陶瓷骨支架及海藻酸钙/双相陶瓷骨支架，检测其微观形态与结构、凝固时间、抗溶解性能、抗剪切力、抗压缩力及细胞相容性。结果与结论：①凝固时间：随着胶体浓度的增大，两种复合支架的初凝时间和终凝时间均有所延长。②扫描电镜：材料均呈多孔状的微观结构，孔隙大小不一。③抗溶解性能：3%、4%、5%、7%的海藻酸钙/双相陶瓷骨支架和7%、10%的壳聚糖/双相陶瓷骨支架，在液体环境中抗溶解性能良好。④力学强度：随着胶体浓度的增大，壳聚糖/双相陶瓷骨支架的抗剪切力、抗压缩力均逐渐增强；海藻酸钙/双相陶瓷骨支架抗剪切力、抗压缩力均逐渐增强，但至7%浓度时，抗剪切力、抗压缩力均降低。⑤细胞相容性：壳聚糖/双相陶瓷骨支架的细胞毒性为0至1级，海藻酸钙/双相陶瓷骨支架的细胞毒性为2至3级。⑥结果表明，壳聚糖/双相陶瓷骨支架具备良好的机械性能及细胞相容性。