BACKGROUND: There are still no applicable vascular grafts for clinical replacement of the small-diameter blood vessels (< 6 mm). OBJECTIVE: To explore the possibility of bacterial nano-cellulose (BNC)/chitosan (CH) composite tubes as small-diameter artificial blood vessels. METHODS: BNC/CH tubes were fabricated by introducing chitosan (0.5%, 1%, 2%) into BNC tubes. Physicochemical properties of BNC/CH tubes were analyzed including macro-morphology, microstructure, density, water-holding capacity, burst pressure, mechanical properties, blood compatibility and cytocompatibility. RESULTS AND CONCLUSION: The results indicated that, as compared with the BNC tubes, the water permeability of BNC/CH tubes reduced, the density and water-holding capacity increased, as well as the axial and radial mechanical performance was enhanced, but ductility and elasticity of BNC/CH tubes were weakened. Regarding blood compatibility, hemolysis ratios of BNC and BNC/CH met the requirements of medical grade composites, while the plasma recalcification time showed that anticoagulation of BNC/CH tubes was slightly weaker than that of the BNC tubes. Cell compatibility experiments showed that porcine hip artery endothelial cells stably proliferated on the inner surface of both BNC and BNC/CH tubes, but the proliferation was more obvious on the BNC surface than on the BNC/CH surface.%背景:目前临床上仍旧缺乏人工血管用于小口径血管(< 6 mm)的替换.目的:探究细菌纳米纤维素/壳聚糖复合管作为小口径人工血管的可行性.方法:将培养制备的细菌纳米纤维素管分别与0.5%,1%,2%的壳聚糖复合,制备细菌纳米纤维素/壳聚糖复合管.对这些管材进行宏观形态、微观结构、密度、持水量、爆破压、机械力学性能以及血液相容性和细胞相容性等表征.结果与结论:①与细菌纳米纤维素管相比,细菌纳米纤维素/壳聚糖复合管的水渗透性减小,密度和持水能力均增大,轴向和径向力学性能增强,但是延展性和弹性降低;②对于血液相容性而言,细菌纳米纤维素管和细菌纳米纤维素/壳聚糖管的溶血率均能满足医用材料要求,但是血浆复钙时间测试结果显示,细菌纳米纤维素/壳聚糖管的抗凝性略弱于细菌纳米纤维素管;③细胞相容性实验表明,猪髋动脉内皮细胞在细菌纳米纤维素管和细菌纳米纤维素/壳聚糖管上均能稳定增殖生长,但是在细菌纳米纤维素/壳聚糖管上的增殖效果没有细菌纳米纤维素管明显.
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