短肽水凝胶RADA16结构特征及在生物医学中的应用研究与进展

摘要

背景:与传统生物材料相比,自组装短肽RADA16具有含水量高、结构稳定、生物相容性好及降解产物无毒等优点,近几年在细胞三维培养、组织修复、快速止血及药物/蛋白缓释等生物医学领域具有良好的应用前景.目的:综述短肽水凝胶RADA16基本结构与性能特点及其在生物医学领域的研究进展.方法:应用计算机检索CNKI,Medline及PubMed数据库2005年至2017年关于自组装短肽水凝胶RADA16的文献,中文检索关键词为"自组装短肽水凝胶;RADA16;支架;组织修复;止血;药物/蛋白缓释",英文检索关键词为"self-assembling peptide hydrogel;RADA16;scaffold;tissue repair;hemostasis;drug/protein release ".结果与结论:生理介质或特定盐溶液条件下,自组装肽类分子能够自发形成独特的β-折叠构型,并自组装成纳米纤维,作为新型组织工程支架材料不仅解决了细胞-材料界面不相容的问题,还具备有效模拟细胞外基质、提高细胞生物活性和维持三维环境等优势,RADA16自组装短肽及其衍生肽在三维细胞培养、组织修复、止血以及药物/蛋白缓释等方面展示了良好的开发应用前景,同时也面临诸多挑战,例如如何与新型生物高分子的整合及如何控制损伤靶点等.%BACKGROUND: Compared with traditional biological materials, self-assembling peptide RADA16 has gained much attention in biomedical fields such as three-dimensional cell culture, tissue repair, hemostasis and drug/protein release for its high water content, structural stability, good biocompatibility and nontoxic degradation products. OBJECTIVE: To review the basic structure and properties of self-assembling peptide RADA16 and the latest progress in biomedical research. METHODS: CNKI, Medline and PubMed databases were retrieved by using computer to search relevant articles about self-assembling peptide RADA16 published from 2005 to 2017. The key words were "self-assembling peptide hydrogel;RADA16; scaffold; tissue repair; hemostasis; drug/protein release" in Chinese and English, respectively. RESULTS AND CONCLUSION: Self-assembling peptide molecules can spontaneously form unique β-strand structures and self-assembly into nanofibers under physiological media or salt solution. As a new scaffold material for tissue engineering, it not only solves the problem of incompatibility between cells and material interface, but also has the advantages of simulating the extracellular matrix effectively, enhancing cell biological activity and maintaining three-dimensional environment. The self-assembling peptide RADA16 and its derivatives not only show good prospects for development and application in three-dimensional cell culture, tissue repair, hemostasis, and drug/protein release, but also face many challenges, such as how to integrate the self-assembling peptide with bio-macromolecular material, and how to control the damage to a target.