信号素3A和基质金属蛋白酶-14在非小细胞肺癌中的表达及意义

摘要

目的 检测非小细胞肺癌组织中信号素3A(Semaphorin3A和基质金属蛋白酶-14(MMP-14)的表达关系,探讨联合检测Semaphorin3A及MMP-14对预后的判断价值.方法 94例非小细胞肺癌术后留取的蜡块及临床资料作为观察组,选择80例正常肺组织作为对照组,应用免疫组织化学技术检测二组中Semaphorin3A和MMP-14的表达,分析肿瘤中Semaphorin3A及MMP-14的关系.结果 观察组中Semaphorin3A蛋白表达的阳性率明显低于对照组,观察组中MMP-14表达的阳性率明显高于对照组.观察组中Semaphorin3A及MMP-14表达的阳性率均与肿瘤的胸膜侵犯、淋巴结转移、淋巴结转移个数、分化程度、脉管浸润及PCNA的表达密切相关;Semaphorin3A蛋白的表达与肿瘤的最大直径密切相关;Semaphorin3A及MMP-14的表达具有负相关性,生存分析显示Semaphorin3A及MMP-14的表达与预后相关.结论 非小细胞肺癌组织中Semaphorin3A低表达、MMP-14高表达时促进肿瘤的发生和进展,二者可能具有负向协同作用,术后联合检测Semaphorin3A及MMP-14的表达对判断预后有一定价值.%Objective To investigate the correlation between the expression levels of semaphorin3A and MMP14,and their subsequent prognostic significance in nonsmall cell lung cancer(NSCLC).Methods The expression of semaphorin3A and MMP14 protein levels was analyzed in 94 cases of NSCLC tissues and in 80 cases of normal lung tissues,using immunohistochemistry(IHC).Correlation and survival analysis were used to further investigate their association and prognostic value.The correlation analysis using Pearson test,and log-rank test for survival analysis.Results The NSCLC tissues exhibited a lower expression of semaphorin3A and a higher expression of MMP14 than in the control lung tissues.The downregulation of semaphorin3A and upregulation of MMP14 may promote pleural invasion,lymph node metastasis,vascular invasion and proliferating cell nuclear antigen expression.The expression of semaphorin3A was correlated with the maximum diameter of tumor.There was a negative correlation between the protein expression levels of semaphorin3A and MMP14 in NSCLC tissues.Conclusion The data suggest that lower expression of semaphorin3A and a higher expression of MMP14 may promote occurrence and development in NSCLC and that the combined detection of semaphorin3A and MMP14 protein may be a helpful tool in predicting the prognosis of NSCLC.