多肽BRC4的固相法合成及其与RAD51(231-260)的相互作用

摘要

Three polypeptides(BRC4, BRC4-1 and BRC4-2), with over 90% purity, were synthe-sized by the Fmoc solid synthesis strategy then separation and purification by RP-HPLC, using Wang resin as carrier, Fmoc protected amino acid as material and HOBT/HBTU/DIEA as coupling reagents. The structures were confirmed by MS ( ESI ) . The interactions of BRC4 , BRC4-1 and BRC4-2 with RAD51 were investigated by circular dichroism. The circular dichroism results showed that the se-quence of interaction strength with RAD51(236-260) was BRC4-1>BRC4-2>BRC-4.%采用Fmoc固相合成策略,以Wang树脂为载体,Fmoc保护的氨基酸为原料,HOBT/HBTU/DIEA为缩合剂,经RP-HPLC分离纯化合成了3条多肽(BRC4,BRC4-1和BRC4-2),纯度>90%,其结构经MS(ESI)确证.采用圆二色光谱研究了BRC4,BRC4-1和BRC4-2与蛋白RAD51关键肽段RAD51(231-260)的相互作用.结果表明:3条多肽与RAD51(231-260)的相互作用强度为BRC4-1>BRC4-2>BRC4.