胃癌组织中基质金属蛋白酶-2和胰岛素样生长因子-1蛋白表达及其意义

摘要

Objective To investigate the expression of matrix metalloproteinase-2 (MMP-2) and insulin-like growth factor-1 (IGF-1) in gastric carcinoma and their clinicopathological significance. Methods Expressions of MMP-2 and IGF-1 were examined by using immunohistochemical SP staining and cross- compared with clinicopathological features of gastric carcinoma. Results High expression of MMP-2 and IGF-1 were observed in 70.4% (307/436) and 49. 5% (216/436) of gastric carcinoma tissues respectively, significantly higher than those in non-tumor gastric mocosa(3. 3% and 5.4% , respectively; all P <0.05). The high expression rate of MMP-2 and IGF-1 were significantly associated with the patient age, tumor size, tumor location, Lauren classification, TNM staging, depth of tumor infiltration, presence of vessel invasion, lymph node and distant metastasis ( all P < 0. 05 ). In addition, the expression of MMP-2 was positively linked with the expression level of IGF-1 (P<0.05). Univariate analysis showed that high expression of MMP-2, was significantly associated with poor prognosis of tumor of TNM stage I and II ( all P < 0. 05 ) , high expression of IGF-1 was significantly correlated with poor prognosis of patients with TNM stage Ⅰ , Ⅱ and Ⅲtumor (all P < 0. 05). Cox multivariate analysis indicated that the high expressions of MMP-2 and IGF-1 could be independent prognostic indices for gastric carcinoma. Conclusions High expression of MMP-2 and IGF-1 proteins are significantly correlated with the invasion and metastasis of gastric carcinoma,it is helpful to simultaneously detect the expressions of MMP-2 and IGF-1 proteins in predicting prognosis of patients with gastric carcinoma.%目的 探讨基质金属蛋白酶-2(MMP-2)和胰岛素样生长因子-1(IGF-1)两种蛋白在胃癌组织中的表达及其临床病理意义.方法 应用免疫组化方法检测436例胃癌组织和92例非肿瘤胃黏膜组织中MMP-2和IGF-1蛋白的表达情况,分析MMP-2和IGF-1与胃癌浸润、转移和预后之间的关系.结果 胃癌组织中MMP-2和IGF-1的高表达率分别为70.4%(307/436)和49.5%(216/436),明显高于非肿瘤胃黏膜组织中的3.3%(3/92,P<0.05)和5.4%(5/92,P<0.05),两种蛋白在胃癌组织中的表达水平均与肿瘤患者的年龄、肿瘤大小、肿瘤部位、Lauren分型、TNM分期、浸润深度、脉管侵犯、远处转移和淋巴结转移存在相关性(P值均<0.05);MMP-2和IGF-1两种蛋白的表达呈正相关(P<0.05);按TNM分期的单因素生存分析表明,MMP-2高表达与Ⅰ～Ⅱ期胃癌患者的预后不良相关(P值均<0.05),IGF-1高表达与Ⅰ～Ⅲ期胃癌患者的预后不良有关(P值均<0.05);MMP-2和IGF-1的高表达均为胃癌的独立预后因素.结论 MMP-2和IGF-1参与了胃癌的侵袭、转移过程,同时检测MMP-2和IGF-1两种蛋白的表达有助于判断胃癌患者预后.