DNA Interaction and Cytotoxic Activity of a Chiral Amino-alcohol Schiff Base Derived Cu(Ⅱ) Complex

摘要

A novel copper(Ⅱ) complex based on chiral amino-alcohol derived Schiff base ligand,[Cu_4(R-L)_4(H_2O)_2]·(CH_3COOH)_2·(H_2O)(1,(R)-H_2 L =(R)-3-phenyl-2-(2-hydroxy-3-methoxybenzylideneamino) propane-1-ol),was synthesized and characterized by EA,IR,UV-Vis,ESI-MS,circular dichroism spectra and single-crystal X-ray diffraction.Complex 1 crystallizes in orthorhombic,space group Ρ2_12_12 with a = 15.7660(14),b = 49.526(3),c = 10.4213(9) ?,V = 8137.2(12) ?~3,Ζ = 4,C_(72)H_(81)Cu_4N_4O_(19),Mr = 1560.57,μ = 1.096 mm^(-1),F(000) = 3244,Flack = 0.06(3),the final R = 0.0924 and w R = 0.2451(I > 2σ(I)) for 41108 observed reflections.The interactions of the complex with calf thymus DNA(CT-DNA) were investigated by some spectroscopic technique methods.The results show the complex exhibits strong binding with CT-DNA.In addition,in vitro cytotoxicity test of 1 towards four kinds of human cancerous cell lines(He La,HL-60,Caco-2 and A549) showed substantial cytotoxic activity.The experimental investigations indicated that the chirality of complex 1 play an important role in cytotoxicity and interactions with DNA.