注射氢水对一次性力竭运动大鼠骨骼肌氧化应激损伤的影响

摘要

Objective To study the effects of supplementing hydrogen-rich water on the oxidation stress-induced damage in skeletal muscle after single bout of exhaustive exercise. Methods Thirty healthy SD male rats were equally and randomly divided into: sedentary control group (C) ,exercise control group (E) and exercise + hydrogen water group (H) . Intraperitoneal injection of hydrogen-rich water (10 ml·kg-1) was performed for group H, and the same amount of normal saline was intraperitoneally injected to group E one minute before single bout of exhaustive exercise on a treadmill at the speed of 28 m·min-1 with 0° slope. 3 hours after exercise, the gastrocnemius was drawn to determine the density of 3-nitrotyrosine (3-NT) and 8-hydroxy deoxyguanosine (8-OHdG) by Elisa measurement, and MDA, SOD, GSH and TAOC by chemical colorimetry. Results Compared with group C, 3-NT, MDA and 8-OHdG of group E significantly elevated (P ＜ 0.05, P ＜ 0.01,and P ＜ 0.01, respectively) , while SOD and GSH significantly reduced (P ＜ 0.05) , however,TAOC reduced insignificantly; MDA was significantly lower (P ＜ 0.01 ) and 8-OHdG was remarkably higher (P ＜ 0.05) , whereas 3-NT, SOD, GSH and TAOC of group H and C changed significantly.When compared with group E, MDA and 8-OHdG of group H significantly decreased (P ＜ 0.01 and P ＜ 0.05, respectively) , SOD, GSH and TAOC significantly increased (P ＜ 0.01, P ＜ 0.01, P ＜0.05, respectively) , whereas 3-NT changed insignificantly. Conclusion Hydrogen-rich water could alleviate the metabolic level of free radical, elevate the activity of antioxidase, and thus protect stressinduced damage in skeletal muscle after acute exhaustive exercise.%目的:观察注射氢水对一次性力竭运动大鼠骨骼肌氧化应激损伤的影响.方法:30只健康雄性SD大鼠,按体重配对后随机分成安静对照组(C组)、运动对照组(E组)与运动给氢组(H组)3组,每组10只.C组不运动.H组于运动前按10 ml·kg-1体重剂量腹腔注射氢水,E组注射同体积生理盐水,然后以0坡度、28 m·min.速度进行一次性力竭跑台运动,力竭运动后3 h取后肢腓肠肌,用Elisa法测定其3-硝基酪氨酸(3-NT)和8-羟基脱氧鸟苷(8-OHdG)浓度,用化学比色法测定MDA、SOD、GSH与TAOC.结果:与C组比较,E组3-NT、MDA与8-OHdG均显著升高(P<0.05,Jp<0.01,P<0.01),SOD、GSH含量显著降低(Jp<0.05),TAOC有所降低,但无统计学意义;H组3.NT、SOD、GSH、TAOC与C组比较无显著性差异,MDA显著低于C组(P<0.01),8-OHdG浓度显著高于C组(P<0.05).H组3.NT与E组比较无明显变化,但MDA、8-OHdG显著降低(P<0.01,P<0.05),SOD、GSH、TAOC显著升高(P<0.01,P<0.01,Jp<0.05).结论:氢水能降低骨骼肌自由基代谢水平,提高骨骼肌抗氧化酶活性,增强骨骼肌的总抗氧化能力,对骨骼肌运动性氧化应激损伤具有保护作用.