连接酶链反应检测尿液中的淋病双球菌

摘要

Objective: To evaluate the value of ligase chain reaction(LCR) in the diagnosis of diplococcus gonorrhoeae in urine.Methods: LCR detection of the urine for Neisseria gonorrhoeae and bacteria culture of discharge was performed simultaneously to 276 patients with urethritis or cervicitis seeking treatment in sex transmitted diseases (STDs) outpatient clinic. For specimens with discordant results, polymerase chain reaction was conducted. The purpose was to detect the respective sensitivity and specificity of bacteria culture and LCR.Results: 24 of 276(8.7%) patients had positive LCR results and 21 of 276(7.6%) were positive for culture.5 specimens had discordant results from LCR and bacteria culture. The sensitivity and specificity of LCR in the diagnosis of gonorrhoeae were 92.3% and 100% respectively.Conclusion: This study showed that LCR had a higher sensitivity and specificity for the diagnosis of gonorrhoeae from urine.%目的 评价连接酶链反应(LCR)技术对尿液中淋病双球菌诊断的意义.方法 对在STD门诊就诊的276例尿道/宫颈炎患者进行分泌物细菌培养及尿液淋球菌LCR检测,对两项结果不符合的标本进行聚合酶链反应(PCR)检测,确定细菌培养、LCR法检测尿液中淋球菌的敏感性、特异性.结果 276例患者中24例LCR检测阳性(8.7％),21例培养阳性(7.6％).5例两项结果不符合者进行聚合酶链反应检测.结果表明LCR的敏感性及特异性分别为92.3％,100％.结论 LCR分析法检测尿液中的淋球菌具有较高的敏感性及特异性.