To understand physiological effects of rapid cold hardening (RCH) on the diapause larvae of the rice stem borer, Chilo suppressalis Walker, at physiological and biochemical levels, the contents of water, lipid, total sugar, low-molecular weight carbohydrates and the actitives of SOD, POD, CAT in the diapause larvae were measured. The results showed that the content of water in RCH-treated larvae was significantly lower than that under the check at 0. 05 probability levdl, while the activity of POD was in adverse. The contents of lipid, total sugars, trehalose, glucose, glycerol and the activities of SOD, CAT showed no significant difference. The content of water of larvae under non-rapid cold hardening(NRCH) was significantly lower than that under the check, while the acitivity of POD was in adverse and the. Rest showed no significant difference. The activities of POD of RCH larvae were just significantly higher than those of NRCH-treated larvae (P<0. 05). Therefore, RCH could enhance physiological effects of water and POD on the diapause larvae.%为从生理生化水平上探讨快速冷耐受(RCH)对二化螟滞育幼虫的生理效应,分别对正常饲养(CK)、非快速冷耐受(NRCH)和RCH处理过的二化螟幼虫的水分、脂质、总糖、小分子碳水化合物含量及SOD、POD、CAT活性进行了测定.结果表明,RCH处理组水分含量较对照组显著下降,POD活性对照组显著上升,脂质、总糖、海藻糖、葡萄糖、甘油含量及SOD和CAT活性无显著差异；NRCH处理组水分含量较对照组显著降低,而POD活性则显著下降,其余未见显著变化；RCH处理组仅见POD活性较对照组显著增加.由此可见,RCH处理对二化螟滞育幼虫体内水分和POD诱导效应显著.
展开▼
