转Bt基因水稻对二化螟幼虫取食、生长及其存活的影响

摘要

The Bt transgenic rice line, i.e. KMD1 containing a synthetic cry1Ab gene from Bacillus thuringiensis Berliner was tested in the laboratory for evaluating its effects on larval food consumption, growth and survival of striped stem borer (SSB), Chilo suppressalis (Walker) under different temperatures. The food consumption and body weight growth as well as the survival of both 3rd and 5th instar larvae fed on Bt rice were significantly reduced, as compared with those on the control. The temperature showed no marked effect on both food consumption and body weight growth of larvae fed on Bt rice stems since the onset of 3rd instar, eonversely had significant effects as since the initial stage of 5th instar. In contrast, the temperature did not distinctly influence the survival of both 3rd and 5th instar larvae. There was a linearly positive relationship between the corrected mortality of larvae and the accumulative anmunt of Bt rice tissues ingested by larvae.%在实验室中以转cry1Ab基因水稻-克螟稻1号为材料,研究了不同温度下Bt水稻对二化螟Chilosuppressalis(Walker)3龄和5龄幼虫取食、生长及其存活的影响.结果表明,不同温度下3龄和5龄幼虫取食Bt水稻后,其取食量、体重增长和存活率均极显著低于对照.温度对3龄幼虫取食、生长和存活无显著影响,但对5龄幼虫的取食和体重增长则有显著影响.幼虫死亡率与其取食Bt水稻的累积食量间存在着正相关关系.