通过中子辐射诱变早籼稻品种红矮 B,获得矮秆多分蘖突变体 bf370.该突变体与野生型相比表现为植株矮化,分蘖极多.bf370在全生育期内的分蘖数达200个左右,是野生型分蘖数量的14倍以上.遗传分析表明该矮秆多分蘖突变体表型受一对隐性核基因控制.利用突变体 bf370与日本晴杂交构建的 F2群体将突变基因定位到第1染色体长臂 Indel 4与Indel 10之间398 kb 区域内.测序分析发现,与野生型相比突变体该区段内的 D10基因在第2外显子上缺失66 bp 碱基,导致 D10蛋白 RPE65结构域22个氨基酸缺失.结合 D10其他突变体表型推断,bf370表型极有可能由 D10突变所致.%A dwarf and high-tillering mutant bf370 was obtained from the indica rice variety Hong’ai B by neutron radiation.Compared with the wild type,the mutant bf370 was characterized by dwarf plant and more tillers.The number of tillers in bf370 was about 200,which was 1 3 times more than that in the wild type.Genetic analysis indicated that this phenotype was controlled by a single recessive nuclear gene,which was mapped to a 398 kb region between the marker Indel 4 and Indel 10 on the long arm of chromosome 1 using the F2 mapping population generated from the cross between mutant bf370 and Nipponbare.Sequencing analysis showed a 66 nucleotide deletion in the second exon of D10 ,resulting in a 22 amino acids deletion in the predicted RPE65 domain of D10 protein in the bf370 mutant.Combined with the phenotype of other d10 mutants,the phenotype of bf370 was likely caused by a 66 nucleotide deletion in D10 .
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