Objective To explore the relationship of anti-laminin-1 (anti-LN1 )with abortion and infertility,and to determine the effect of glucocorticoid on fertility in anti-LN1 antibody positive mice. Methods We analyzed abortion rate between BALB/c mice immunized by LN-1 antigen (the model group)and complete Freund’s adjuvant-injected controls.We divided the immune mice into two groups.Dexamethasone (1 .5 mg)was injected in one group every other day for 5 days (the drug group),while the same amount of saline solution was injected in the other group.We measured the level of anti-LN1 antibody in the serum. Results We found that the pregnancy rate of the model group was 8.9%, while the control group one was 90%.Meanwhile,the abortion rate of the model group was 24.4%,while no abortion was observed in the control group one.In addition,we found that the drug group had a pregnancy rate of 20%,while the control group two had 8.9%.Moreover,the abortion rates were 1 0% in the drug group and 33.3% in the control group two.The anti-LN1 antibody levels in the serum of the model group,the control group one and the drug group was (2.557 ± 1 .395),(0.1 06 ±0.005),(2.850 ±1 .1 1 6),respectively,and the difference was not significant. Conclusion Anti-LN1 antibody is related to reproductive barrier.Glucocorticoid has positive effect on immunogenicity of the reproductive barriers in female mice.These results might provide a novel basis for the etiology of abortion.%目的：探讨抗层粘连蛋白-1（LN-1）抗体与小鼠流产、不孕等生殖障碍的相关性及糖皮质激素对改善抗层粘连蛋白-1抗体阳性小鼠生育状况的作用。方法（1）采用 BALB／c 小鼠，以层粘连蛋白-1为抗原构建BALB／c 小鼠免疫模型（模型组），同时以单纯完全弗氏佐剂注射为对照一组，观察两组的流产率和产仔数。（2）选择免疫成功小鼠，随机分成两组，分别于妊娠当日起隔日注射1．5mg 地塞米松（研究组）或等量生理盐水（对照二组）共5次，观察流产情况及产仔率。（3）ELASE 法测定各组血清抗层粘连蛋白-1抗体滴度值。结果（1）对照一组妊娠率为90％，流产率为0；模型组妊娠率为8．9％，流产率为24．4％。（2）应用地塞米松组（研究组）妊娠率为20％，对照二组妊娠率为8．9％，两组流产率分别为10％和33．3％，差异具有统计学意义。（3）模型组、对照一组和地塞米松组（研究组）三组血清抗层粘连蛋白-1抗体值分别为（2．557±1．395）、（0．106±0．005）、（2．850±1．116），模型组与地塞米松组差异无统计学意义，模型组与对照一组、地塞米松组与对照一组差异具有统计学意义。结论（1）抗层粘连蛋白-1抗体与小鼠的生育障碍具有相关性。（2）糖皮质激素在改善雌鼠免疫源性生育障碍方面，可能具有积极的作用，进一步研究有望为流产的病因学提供新的理论基础。
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