目的：观察原花青素对脊髓损伤后大鼠胶质纤维酸性蛋白(GFAP)、脑源性神经营养因子(BDNF)表达变化的影响。方法48只健康成年Sprague-Dawley大鼠分为原花青素组(A组)和对照组(B组)。采用Allen法复制大鼠T9急性脊髓损伤模型，于术后1 d、3 d和7 d对各组大鼠行后肢运动功能BBB评分和斜板试验；测定血清丙二醛(MDA)和超氧化物歧化酶(SOD)；以及免疫组织化学染色检测脊髓GFAP和BDNP的表达。结果术后3 d、7 d，A组大鼠BBB评分和斜板试验成绩均优于B组(P<0.05)。术后1 d、3 d及7 d，A组大鼠血清SOD活性和MDA含量优于B组(P<0.05)。术后各时间点，A组GFAP表达均明显低于B组(P<0.01)；A组BDNF表达均显著高于B组(P<0.001)。结论原花青素可有效抑制脂质过氧化反应，抑制脊髓损伤后大鼠脊髓组织GFAP的表达，促进内源性BDNF的合成，从而促进脊髓损伤大鼠功能恢复。%Objective To observe the effect of proanthocyanidin on the expression of brain-derived neurotrophic factor (GFAP) and brain-derived neurotrophic factor (BDNF) in rats with spinal cord injury (SCI). Methods 48 healthy adult Sprague-Dawley rats were ran-domly divided into proanthocyanidin treatment group (group A, n=24) and control group (group B, n=24). Allen's method was used to estab-lish the model of acute spinal cord injury in T9. 1, 3 and 7 days after operation, 8 rats in each subgroup were assessed with Basso-Beat-tie-Bresnahan (BBB) scale and Slanting Board Test, the serum malondialdehyde (MDA) and superoxide dismutase (SOD) were detected, and the expression of GFAP and BDNF of the spinal cord were detected with immunohistochemistry. Results The results of BBB scale and Slanting Board Test were better in group A than in group B 3 and 7 days after SCI (P<0.05). The levels of SOD and MDA were better in group A than in group B 1, 3 and 7 days after SCI (P<0.05). The expression of GFAP was lower, and the expression of BDNF was higher in group A than in group B all the time points after SCI (P<0.01). Conclusion Proanthocyanidin can inhibit the lipid peroxidation and the ex-pression of GFAP in spinal cord after SCI, stimulate the synthesis of endogenous BDNF, and improve the motor function in rats after SCI.
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