目的：观察经皮电刺激对脊髓损伤后大鼠胶质纤维酸性蛋白(GFAP)、核因子-κB(NF-κB)及白细胞介素-6(IL-6)表达的影响。方法90只健康成年Sprague-Dawley大鼠分为正常对照组(A组, n=30)、经皮电刺激组(B组, n=30)和对照组(C组, n=30)。采用Allen法复制大鼠T9急性脊髓损伤模型，于术后1 d、3 d和7 d对各组大鼠行后肢运动功能BBB评分和斜板试验；免疫组织化学染色检测脊髓GFAP、NF-κB及IL-6的表达。结果术后3 d、7 d，B组大鼠BBB评分和斜板试验成绩均明显优于C组(t>3.349, P<0.01)。术后各时间点，B组GFAP、NF-κB及IL-6表达均显著低于C组(t>20.815, P<0.001)。结论经皮电刺激可有效抑制炎症反应，抑制脊髓损伤后大鼠脊髓组织GFAP的表达，从而促进脊髓损伤大鼠运动功能恢复。%Objective To observe the effect of transcutaneous electrical stimulationon on Jiaji acupoint (Ex-B 05) on the expression of glial fibrillary acidic protein (GFAP), nuclear factor kappa B (NF-κB) and interleukin-6 (IL-6) in rats with spinal cord injury (SCI). Meth-ods Ninety healthy adult Sprague-Dawley rats were randomly divided into normal group (group A, n=30), transcutaneous electrical stimula-tion treatment group (group B, n=30) and control group (group C, n=30). Allen's method was used to establish the model of acute SCI in T9. All of them were assessed with Basso-Beattie-Bresnahan (BBB) scale and Slanting Board Test, and the expression of GFAP, NF-κB and IL-6 of the spinal cord were detected with immunohistochemistry one, three and seven days after operation. Results The results of BBB scale and Slanting Board Test were better in group B than in group C three and seven days after SCI (t>3.349, P<0.01). The expression of GFAP, NF-κB and IL-6 was lower in group B than in group C at all the time points after SCI (t>20.815, P<0.001). Conclusion Transcutane-ous electrical stimulation can inhibit the inflammation and the expression of GFAP in spinal cord after SCI, and improve the motor function in rats after SCI.
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