目的:将氧化石墨烯加入壳聚糖/p-甘油磷酸钠温敏凝胶中,制备石墨烯/壳聚糖/β-甘油磷酸钠复合温敏凝胶,表征其基本理化性质并对生物学特性进行初步评价.方法:将氧化石墨烯(graphene oxide,GO)加至壳聚糖/β-甘油磷酸钠(chitosan/β-glycerophosphate,CS/GP)温敏凝胶体系中,根据GO与CS不同的质量分数,制备了1wt％GO/CS/GP和3wt％GO/CS/GP复合温敏凝胶,以CS/GP温敏凝胶为对照,表征了各凝胶成胶时间、化学结构变化、微观结构和吸水率.将小鼠前成骨细胞MC3T3-E1接种于凝胶中,SEM观察细胞接种24h的粘附现象,CCK-8法检测细胞的增殖,并用荧光倒置显微镜观察细胞的生长情况.结果:凝胶的成胶时间随GO含量增加而缩短,CS/GP的平均成胶时间为(9.05±0.21)min,1wt％GO/CS/GP的平均成胶时间为(6.60±0.22)min,3wt％GO/CS/GP平均成胶时间为(4.50±0.18)min;GO和CS可通过酰胺键结合;凝胶均呈现出均一的相互通联的支架结构,1wt％GO/CS/GP复合温敏凝胶具有更大孔径(44.8±4.3μm)和吸水率(527±21％)以及更好的促成骨细胞增殖生长特性.结论:相比于壳聚糖/β-甘油磷酸钠温敏凝胶,石墨烯/壳聚糖/β-甘油磷酸钠复合温敏凝胶具有更好的理化性质和生物学特性.%Objective:To prepare graphene/chitosan/β-glycerophosphate hydrogel by adding GO into chitosan/β-glycerophosphate thermal-sensitive hydrogel and evalvuate its physiochemical and basic biological properties.Methods:GO was added into chitosan/β-glycerophosphate hydrogel system,and lwt％GO/CS/GP hydrogel and 3wt％GO/CS/GP hydrogel were prepared by changing the weight fraction of GO and CS.The gelling time,chemical structure,microstructure and water absorption of the hydrogels were evaluated.MC3T3-E1 was cultured on the hydrogels,the cells' adhesion was observed by SEM,the cells' proliferation was analyzed by CCK-8 method,and the cells' living state was observed by inverted fluorescence microscope.Results:GO/CS/GP hydrogels took less time to gel,the average gelling time of CS/GP,1wt％ GO/CS/GP and 3wt％GO/CS/GP hydrogels were (9.05± 0.21)min,(6.60± 0.22)min and (4.50± 0.18)min.Amido bond can be found in GO/CS/GP hydrogels.All the gels were presented a harmonious interconnected net-structure.The average pores size of 1wt％GO/CS/GP hydrogel was (44.8t 4.3)μm and water absorption of 1wt％GO/CS/GP hydrogel was (527± 21)％,both were the biggest among the hydrogels.And 1wt％GO/CS/GP hydrogel was more potential to promote the proliferation ofMC3T3-E1.Conclusion:Compared with CS/GP thermal-sensitive hydrogel,lwt％GO/CS/GP thermal-sensitive composite hydrogel has better physiochemical and biological properties.
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