哮喘患儿外周血Th17细胞CD4+CD25+调节性细胞变化及其与病情相关分析

摘要

Objective To observe the levels of Thl7 cells, CD4+CD25+ regulatory T cells in the asthmatic children and relationship between the two types of cells and children' s condition. Methods Flow cytometry was used to detect the percentages of the Thl7 cells and CD4+CD25+ regulatory T cells in the peripheral blood of acute asthma children (asthma group, n - 60) .alleviated period asthma children (n = 30) and healthy children (healthy control group, n = 30).Acute asthma children were divided into 3 groups: mild, moderate and severe asthmatic patients. Results Compared with the healthy control group (1.02% ± 0.28%) and alleviated period asthma children (1.65% ± 0.38%), the numbers of CD4+ cells (Thl7) expressing IL-17 in peripheral blood of acute asthma(2.24% ± 1.02%) were increased, and the differences were statistically significant (P<0.05). The levels of CD4+CD25+T cells in peripheral blood of acute asthma and alleviated period asthma children (5.37% ± 0.80% ; 6.05% ± 0.87%) were significantly lower than those of healthy children (7.11% ± 0.89%) (P < 0.05). Thl7 cells were positively correlated with the course of childhood asthma(r = 0.649, P < 0.05).CD4+CD25+ regulatory T cells were negatively correlated with the course of childhood asthma (r =-0.599, P < 0.05). Conclusion The immunization response of Thl7 cells in peripheral blood of asthmatic children is strengthened, but the immune function of CD4+CD25+regulatory T cells is decresed. The severity of asthma is closely related to the inbal-ance of Th71ATreg cellular immunity.%目的 探讨支气管哮喘患儿外周血中辅助T细胞(Th) 17细胞和CD4+ CD25+调节性T细胞(Treg)的变化与儿童哮喘病情的相关性.方法　收集2009年月5月至2010年4月于郑州大学第一附属医院就诊的患儿,均为首次确诊哮喘或规范吸入激素停用＞3个月后复发及近1个月内无明显感染者.采用流式细胞仪测定患儿外周血中Th17细胞及CD4+CD25+Treg比例的变化.结果 Th17细胞在哮喘急性期组(2.24％±1.02％)较哮喘缓解期组(1.65％±0.38％)及健康儿童组(1.02％±0.28％)均显著增高(P＜0.05),哮喘缓解期组(1.65％±0.38％)和健康儿童组(1.02％±0.28％)无明显差别,CD4+CD25+Treg细胞比例在3组儿童间差异均有统计学意义(F=45.604,P＜0.05),与健康儿童组(7.11％±0.89％)相比,哮喘缓解期组(6.05％±0.87％)和哮喘急性期组(5.37％±0.80％)的比例明显下降,而哮喘急性期组较健康儿童组下降.哮喘急性期组轻、中、重度3组之间差异同样有统计学意义.Th17细胞与哮喘患儿病情呈正相关(r=0.649,P＜0.05),而CD4+CD25+Treg细胞与病情呈负相关(r=-0.599,P＜0.05).结论 急性期哮喘患儿Th17免疫应答增强,急性期患儿、缓解期CD4+CD25+Treg细胞免疫功能下降,哮喘的严重程度与外周血中的Th 17/Treg细胞免疫失衡密切相关.