目的 探讨芹菜素对人胶质瘤细胞的抑制作用及其分子学机制.方法 人胶质瘤U 87细胞给予0、20、40μm o l/L芹菜素,通过MTT评价细胞增殖,流式细胞仪检测细胞凋亡.进一步U87细胞转染Bak1 siRNA,24 h后给予芹菜素刺激,体外观察细胞的增殖和细胞凋亡情况,Western blot检测Bak1表达.结果 芹菜素可有效抑制胶质瘤细胞生长,诱导其凋亡.抑制Bak1表达后,芹菜素抑制胶质瘤增殖能力下降,未见细胞发生凋亡.结论 芹菜素抑制胶质瘤细胞生长促进其凋亡是通过活化Bak1蛋白实现的.%Objective To explore the inhibitory effect of apigenin on human glioma and its molecular mechanism .Methods Human glioma U87 cells were treated with 0 ,20 ,and 40 μmol/L apigenin .Cell proliferation was evaluated by MTT ,and apoptosis was detected by flow cytometry .U87 cells were further transfected with Bak1 siRNA ,and stimulated with apigenin after 24h .The proliferation and apoptosis of the cells were observed in vitro ,and the expression of Bak1 was detected by Western blot .Results Apigenin can effectively inhibit the grow th of glioma cells and induce apoptosis .After inhibiting the expression of Bak1 ,apigenin inhibited the proliferation of glioma cells ,and no cell apoptosis occurred .Conclusion Apigenin inhibits glioma cell growth and pro-motes apoptosis by activating Bak1 protein .
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