人雌激素硫酸基转移酶基因在雌激素依赖型子宫内膜腺癌组织中的表达及临床意义

摘要

目的 探讨人雌激素硫酸基转移酶(NEST)基因与雌激素依赖型子宫内膜腺癌发生、发展关系,为其病因学研究提供有意义的帮助.方法 采用Western-Blot及RT-PCR方法分别检测中国医科大学附属盛京医院妇科2005年7月至2007年10月40例子宫内膜腺癌组织,20例正常子宫内膜组织、12例不典型增生子宫内膜组织中NEST蛋白及mRNA表达的相对含量.结果 NEST蛋白及mRNA在正常子宫内膜增殖期、分泌期和萎缩内膜组织中的表达差异显著(P<0.01).NEST蛋白及mRNA在正常子宫内膜组织、不典型增生子宫内膜组织及子宫内膜腺癌组织中的表达含量逐渐降低,三者比较有差异(P<0.01).hEST mRNA和蛋白含量与子宫内膜腺癌组织学分级、肌层浸润深度、手术病理分期及淋巴结转移等无关(P>0.05).子宫内膜腺癌组织中,NEST mRNA和蛋白在ER阳性组织中的含量明显高于ER阴性组织,差异显著(P<0.01).结论 NEST表达降低或缺失可能与雌激素依赖型子宫内膜腺癌的早期形成有一定的关系,但NEST不能作为子宫内膜腺癌病情程度和预后评价的指标.%Objective To research relationship between the occurrence and development of endometrial adenocarcinoma, to provide significant assistance to studies on etiology of endometrial adenocarcinoma. Methods Inspect the protein expression of hEST by Western-Blot test, inspect the expression of the mRNA of the hEST by RT-PCR, comparing the difference in different tissues. Results The content of hEST protein and mRNA expression in normal endometrial proliferation, secretory period and shrink endometrial tissue was significantly (P <0.01 ). The content of hEST protein and mRNA expression in normal endometrium was the high, and decreased inatypical hyperplasia and endometrial adenocarcinoma tissues. The difference among the three groups was very marked ( P < 0.01). In endometrial adenocarcinoma tissues with different clinical stage, pathological, muscular invasion and so on, the expression level of the mRNA and protein of hEST were of no difference ( P > 0. 05 ). In endometrial adenocarcinoma, hEST mRNA and protein in the ER-posi-tive was significantly higher than the ER-negative with significant differences (P < 0.01). Conclusions hEST may be related with the tumorigenesis of estrogen-dependent endometrial adenocarcinoma,but hEST wasn't index for endometrial adenocarcinoma.