P57kip2和Maspin在病理性瘢痕组织中的表达

摘要

Objective To study the expression of P57kip2 and Maspin in the pathological scar and their possible role in the pathogenesis of abnormal scars.Methods Immunohistochemistry integrated image analysis and reverse transcriptionpolymerase chain reaction (RT-RCR) were performed to detect the expression of P57kip2 and Maspin in hypertrophic scar,keloid,mature scar and normal skin.Statistics was used to analyze the datas.Results The expression of P57kip2 protein was fixed to fibroblast intranuclear in abnormal scar,and the expression of P57kip2 protein and P57kip2 mRNA decreased ( P ＜ 0.05 ).The expression of Maspin protein was fixed to fibroblast cytoplasm and intranuclear in abnormal scar,and the expression of Maspin protein and Maspin mRNA decrease,compared with that in normal group ( P ＜0.05).There was positive correlation between P57kip2 protein and Maspin protein expression( P ＜ 0.01 ).Conclusions The decreased expression of P57kip2 and Maspin in abnormal scar shows that they are cicatrix-related genes.There is a positive relationship between the two genes.It may be one of the mechanisms of pathogenesis of abnormal scar.It makes effect through fibroblasts.%目的 研究P57kip2和Maspin在病理性瘢痕组织中的表达情况及相互关系,探讨它们在病理性瘢痕形成中的作用及机制.方法 应用免疫组化SP法结合计算机病理图像分析和逆转录聚合酶链反应( RT-PCR)检测正常皮肤、成熟瘢痕、增生性瘢痕和瘢痕疙瘩组织中P57kip2和Maspin的表达并对其表达进行统计学分析.结果 病理性瘢痕组织中P57kip2蛋白的表达定位于成纤维细胞的细胞核内,且P57kip2蛋白及mRNA的表达减少,与正常皮肤、成熟瘢痕对照组比较差异有统计学意义(P＜0.05).病理性瘢痕组织中Maspin蛋白的表达定位于成纤维细胞的细胞质和细胞核内,且Maspin蛋白及mRNA的表达减少,与正常皮肤、成熟瘢痕对照组比较差异有统计学意义(P＜0.05).P57kip2与Maspin蛋白表达存在明显正相关(P＜0.O1).结论P57kip2与Maspin在病理性瘢痕组织中均表达减少,是病理性瘢痕相关基因,两者存在明显正相关,是病理性瘢痕的形成机制之一.P57kip2和Maspin可能通过成纤维细胞在病理性瘢痕的形成过程中发挥着重要作用.