Objective To investigate the effect and safety of hair removal via subcutaneous route with IPL in expanded flap.Methods A total of 14 experimental zones were established in two healthy Guizhou mini-pigs with dark hair and white skin.All these skin zones were allocated in seven groups:group 1-6 underwent skin soft tissue expansion(stage Ⅰ:implantation of expander,stage Ⅱ:removal of expander).The subcutaneous tissue in group 1 received IPL irradiation of common level during stage Ⅰ and Ⅱ of skin expansion,group 2 received irradiation of high level during stage Ⅰ,group 4 received irradiation of common level during stage Ⅱ,group 5 received irradiation of high level during stage Ⅱ,group 6 received irradiation of low level during stage Ⅰ and Ⅱ as well as flap trim during stage Ⅱ,group 3 received no irradiation,and the skin in group 7 received irradiation of common level through epidermal route during stage Ⅰ and Ⅱ.Treatments were conducted using a contact tip with IPL(λ =650-950 nm) radiant exposures of different fluencies (common level 18 J/cm2,high level 22 J/cm2,and low level 15 J/cm2) with single pulsed duration of 50 ms.The temperature of tissue surface was determined by infrared thermograph.The microcirculation of expansion flap was determined with PeriFlux System.Digital images were taken for the calculation of hair density count by software Image J.Results The temperature of tissue surface received IPL irradiation decreased soon within 2 minutes.The microcirculation of skin expansion flap in group 1,2,3,6 were all restrained,which began to restore within 4 hour post operation.The hair density in group 1 was the lowest with hair removal rate of 79.17％ ± 2.81％,which was higher than the others significantly(P ＜ 0.05).The hair removal rate in group 6 was 72.95％ ± 13.10％.The hair removal rates in group 4 and 5 were lower than 40％.The hair removal rates in group 7 was 67.38％ ± 7.10％.Conclusions IPL irradiation via subcutaneous route may restrain microcirculation of expansion flap transiently,which was reversible.The hair removal rates can reach above 80％ over 6 months post treatment.It indicates that IPL irradiation via subcutaneous route might be a safe and effective hair removal methods,which may be more effective when it is initiated before the implantation of skin expander%目的 观察在动物实验中,从扩张皮瓣组织面进行强脉冲光照射脱毛的安全性和有效性.方法 选用黑毛白皮贵州小型香猪2只,每只背部分成7个治疗区,相同位置的治疗区为1组.1～6组行皮肤扩张术,1组于皮肤扩张术一、二期接受组织面强脉冲光常规参数照射,2组接受一期组织面高参数照射,4组接受二期组织面常规参数照射,5组接受二期组织面高参数照射,6组接受一、二期组织面低参数及二期手术修剪,3组为单纯扩张组,7组为非扩张组但于一、二期相应时间点行皮面常规参数照射.脱毛治疗参数:单脉冲,脉宽50 ms,能量密度15 ～ 22 J/cm2.对皮瓣进行观察,采用Image J图像分析软件计算脱毛率.利用红外热成像仪检测组织温度,激光多普勒血流仪测量皮瓣血运变化情况.HE染色观察脱毛后毛囊的组织学变化.结果 组织面照射后1～2 min温度即可降至安全范围,照射后即刻即引起血流下降,4h后开始恢复,皮瓣均无血运障碍,成活良好.第1组6个月毛发密度最低,脱毛效率79.17％ ±2.81％,与其他组相比差异具有统计学意义(P＜0.05).第6组次之,脱毛率72.95％±13.10％.第2组术后6个月脱毛效率64.15％±3.68％.第4、5组效果较差,脱毛率均不足40％,第7组脱毛效率67.38％±7.10％.结论 在动物实验中从组织面进行强脉冲光照射虽然会对扩张皮瓣血液循环造成影响,但变化可逆,不影响皮瓣最终血运和成活,2次强脉冲光治疗6个月后皮瓣脱毛率可达80％.组织面治疗效果优于皮面照射途径的效果,埋置扩张器前启动组织面脱毛效果可能更好.
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