TNF-α和PCNA在聚丙烯酰胺水凝胶注射隆乳术后乳腺组织中的表达

摘要

Objective To study the expression of TNF-α and PCNA in human breast tissue with polyacrylamide hydrogel(PAHG) injection,and to provide the initial theory basis for its prognosis and clinical treatment.Methods Immunohistochemistry was used to measure the expression of tumor necrosis factor αt(TNF-ot)and proliferating cell nuclear antigen (PCNA) in 20 normal breast tissues and 40 cases with PAHG injection,analysis was also done by HE staining.Results ①HE staining showed that there were a large number of homogeneous amorphous gel-like injections under optical microscope.Around PAHG there were different degrees of fibrous tissue hyperplasia with or without fibrous degeneration and lots of inflammatory cells.Local foreign body giant cell reaction and ductal dilatation also can be seen around PAHG.②The IA levels of TNF-α and PCNA in the experimental group were 3.9 ± 0.3 and 3.2 ± 0.2,the IA levels of TNF-α and PCNA in the control group were 1.0 ± 0.1 and 1.3 ± 0.3,the IA level in the experimental group was significantly higher than that in the control group (P =0.000).The difference was statistically significant.The positive rates of TNF-t and PCNA in the experimental group were 90％ (36/40) and 85％ (34/40)respectively;the positive rates of TNF-α and PCNA in the control group were 30％ (6/20),40％ (8/20).The positive expression rate in the experimental group was significantly higher than that in the control group (P =0.000).The difference was statistically significant.TNF-α and PCNA has Pearson positive relevance in the changes (r =0.3,P =0.040),the difference was statistically significant.Conclusions TNF-t and PCNA are involved in the immunoreaction of mammalian breast tissue injected with polyacrylamide hydrogel,which may induce the aseptic inflammation,fibrous tissue hyperplasia and related issues through mutual influence.%目的 研究肿瘤坏死因子-α(TNF-α)和细胞核增殖因子(PCNA)在聚丙烯酰胺水凝胶(polyacrylamide hydrogel,PAHG)注射隆乳术后乳腺组织中的表达,为临床治疗及预后提供基础理论依据.方法 试验组为PAHG注射隆乳术后乳房病变组织40例,对照组为正常乳腺组织20例,采用免疫组化法检测TNF-α和PCNA在2组乳腺组织中的表达,结合常规HE染色切片进行分析.结果 ①HE染色结果:试验组光镜下见切片中含大量均质无定形凝胶样注射物,周围有不同程度纤维组织增生,伴纤维组织玻璃样变性及炎性细胞浸润,局部呈异物巨细胞反应,部分乳腺导管扩张.②免疫组化结果:TNF-α和PCNA在试验组中的累积吸光度A值分别为3.9±0.3、3.2±0.2,TNF-α和PCNA在对照组中的累积吸光度A值分别为1.0±0.1、1.3 ±0.3,试验组明显高于对照组,差异有统计学意义(P =0.000).TNF-α和PCNA在试验组阳性表达率分别为90％(36/40)、85％(34/40),TNF-α和PCNA在对照组阳性表达率分别为30％(6/20)、40％ (8/20),试验组阳性表达率明显高于对照组,差异有统计学意义(P =0.000).TNF-α和PCNA表达呈正相关(r=0.3),差异有统计学意义(P =0.040).结论 TNF-α和PCNA参与了聚丙烯酰胺水凝胶注射隆乳者乳房病变组织的免疫反应过程,可能通过相互影响而共同诱导病变部位炎性反应、纤维组织增生等反应的发生.