高效液相色谱法测定首乌丸中2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量

摘要

Objective Determine of 2,3,5,4' -Tetrahydroxystilbene -2 -O -β -D -glucoside in Shouwu pills. The method of determine 2,3,5,4'- Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills was established. Methods The samples were separated at 30℃ on a Ultimate XB-C18 column(4.6mm x 250 mm, 5|xm). When 2,3,5,4'-Tetrah-ydroxystilbene-2-O-β-D-glucoside was eluted with acetonitrile and water(25:75). The detection wavelength was 320nm.The flow rate was all lml'min"1. Results The content of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shouwu pills was 3.895~4.136mg·g-1. Conclusion The method was stable and convenient. This method was suitable for the quality control of Shouwu pills.%目的 高效液相法测定测定首乌丸中2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷成分含量；方法 采用RP-HPLC,色谱柱Ultimate XB-C18(4.6mm×250mm,5μm)；流动相为乙腈-水(2575)；检测波长:320nm;流速:1mL· min-1；柱温30℃.结果 3批首乌丸中2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量为3.895～4.136mg·g-1；结论 该法稳定、简便,适用于首乌丸制剂质量控制.