化湿汤结合润肤止痒膏对湿疹小鼠细胞免疫功能的影响

摘要

目的：观察湿疹小鼠动物模型外周血中的 CD4+和 CD8+的水平，分析化湿汤结合润肤止痒膏对湿疹小鼠CD4+、CD8+及其比值的影响，探讨化湿汤结合润肤止痒膏治疗湿疹的机制。方法将60只健康成年昆明种小鼠随机分为空白对照组、湿疹模型组、湿疹阳性药物组、化湿汤组、润肤止痒膏组及化湿汤结合润肤止痒膏组，除空白对照组外，其余各组建立湿疹模型。空白对照组和湿疹模型组不进行治疗；湿疹阳性药物组用醋酸曲安奈德益康唑乳膏治疗，而化湿汤组、润肤止痒膏组及化湿汤结合润肤止痒膏组则采用相应药物治疗，每日1次。通过流式细胞仪对小鼠外周血中 T 淋巴细胞亚群进行测定。结果与湿疹模型组相比，湿疹阳性药物组、化湿汤组、润肤止痒膏组及化湿汤结合润肤止痒膏组小鼠外周血中 CD4+ T 淋巴细胞百分比及 CD4+ T/CD8+ T 均明显提高（P 均＜0.01），CD8+ T淋巴细胞百分比显著下降（P 均＜0.01）。与湿疹阳性药物组相比，化湿汤组、润肤止痒膏组小鼠外周血 CD4+ T 淋巴细胞百分比及CD4+ T/ CD8+ T 均明显降低（P 均＜0.01），CD8+T 淋巴细胞百分比显著升高（P 均＜0.01）；而化湿汤结合润肤止痒膏组小鼠外周血 CD4+ T 淋巴细胞、CD8+ T 淋巴细胞百分比及 CD4+T/ CD8+ T 无差异（P ＞0.05）。与化湿汤组、润肤止痒膏组相比，化湿汤结合润肤止痒膏组对小鼠外周血中 CD4+T 淋巴细胞百分比、CD8+T 淋巴细胞百分比及 CD4+T/ CD8+T的作用有显著差异（P＜0.01或＜0.05）。结论化湿汤结合润肤止痒膏可能是通过提高 CD4+T百分比，降低 CD8+ T淋巴细胞百分比以及提高 CD4+/CD8+，纠正了 CD4+T 淋巴细胞相对减少，CD8+T 淋巴细胞百分比相对增多，恢复了湿疹小鼠的细胞免疫功能和纠正了免疫失调。%Objective To observe the peripheral blood CD4+ and CD8+ of animal models in mice with eczema, analyze the influence of Huashi decoction combined with Runfuzhiyang cream for CD4+, CD8+ and its ratio in mice with eczema and explore the mechanism of Huashi decoction combined with Runfuzhiyang cream. Methods 60 healthy adult Kunming mice were randomly divided into blank control group, eczema model group, eczema positive drug group, Huashi decoction group, Runfuzhiyang cream group and Huashi decoction combined with Runfuzhiyang cream group, eczema models were established in all groups except for the blank control group. Blank control group and eczema model group were not given treatment. Eczema positive drug group was treated by triamcinolone acetonide acetate econazole cream. Huashi decoction group, Runfuzhiyang cream group and Huashi decoction combined with Runfuzhiyang cream group were treated by corresponding drug, 1 times daily. The T lymphocyte subpopulation in the mice peripheral blood was determined by flow cytometry. Results Compared with eczema model group, mice peripheral blood CD4+ T lymphocytes' percentage and CD4+/CD8+ T significantly increased (P all0.05) in Huashi decoction combined with Runfuzhiyang cream group. Compared with Huashi decoction group and Runfuzhiyang cream group, mice peripheral blood CD4+ T lymphocytes' percentage, CD8+ T lymphocytes' percentage and the effect of CD4+ T/CD8+ T had significant difference (P<0.01 or P <0.05) in the Huashi decoction combined with Runfuzhiyang cream group. Conclusion Huashi decoction combined with Runfuzhiyang cream may restore the cellular immune function and correct the immune disorders in mice with eczema by increasing the percentage of CD4+T, reducing the percentage of CD8+T lymphocytes and increasing CD4+/CD8+to correct the CD4+T lymphocytes decrease relatively and CD8+T lymphocytes' percentage increase relatively.