氯化镧水杨酸和8-羟基喹啉配合物La(C7H5O3)2·(C9H6NO)对粟酒裂殖酵母细胞生长和胞质分裂的影响

摘要

OBJECTIVES To explore the inhibitory effect of lanthanum and salicylic acid and 8-hydroxyquinoline complexes[ La( C7H5O3 ) 2· ( C9H6NO), La(Sal) 2 (Qu) ] on growth and cytokinesis in Schizosaccharomyces pombe. METHODS S. pombe was treated with La (Sal) 2 (Qu) 2, 4,6, 8 and 10 mg·L-1 under constant temperature shaking culture and solid culture condition. The proliferation was detected by MTT assay. The inhibition of cytokinesis of La (Sal)2 (Qu) on S. pombe was observed by cytokinesis inhibition test. DNA content was measured by flow cytometry.RESULTS Compared to normal control group, La(Sal)2(Qu) 6, 8 and 10 mg·L-1 inhibited S. pombe growth under constant temperature shaking culture condition, and decreased cell viability from ( 104.9 ± 8.4) ％ to (36.1 ± 2.2) ％, (35.2 ± 2.6 ) ％ and ( 37.6 ± 2.8) ％, respectively.La( Sal)2(Qu) 4 mg·L-1 inhibited the cell division. Not only did cells with 1 septum significantly increase(29％ ), but cells with 2 or 3 septas were seen. La( Sal)2 (Qu) 2, 4 and 6 mg· L-1 increased DNA content from 1 N to 2 N. CONCLUSION La(Sal)2(Qu) inhibits the cytokinesis and cell growth of S. pombe.%目的 探讨氯化镧水杨酸8-羟基喹啉配合物[La(Sal)2(Qu)]对粟酒裂殖酵母(S.pombe)的生长及细胞分裂的影响.方法 La(Sal)2(Qu) 2,4,6 8和10 mg·L-1处理细胞.应用恒温振荡培养和固体培养MTT法观察La(Sal)2(Qu)对S.pombe生长的影响;胞质分裂抑制实验观察La(Sal)2(Qu)对S.pombe细胞形态和胞质分裂的影响;流式细胞术检测对细胞DNA含量的影响.结果 La(Sal)2(Qu) 6,8和10 mg·L-1在恒温振荡培养S.pombe 8 h,可抑制S.pombe细胞的生长,生长率从正常对照组的(104.9±8.4)%分别降低至(36.1±2.2)%,(35.2±2.6)%和(37.6±2.8)%(P<0.01).La(Sal)2(Qu) 4 mg·L-1可抑制细胞分裂,不仅具有1个隔膜的细胞数目明显增多(28.97%),而且可见具有2个或3个隔膜的细胞;La(Sal)2(Qu) 2,4和6 mg·L-1使细胞DNA含量从1 N增加到2 N.结论 La(Sal)2(Qu)可影响S.pombe细胞的生长和胞质分裂.