AIM: To investigate the protective effect of five naturalanti-oxidants (schisandrin B, Sch B; silibinin, SIB; propyl gallate, PG; sodium ferulate, SF; total flavonoids of hippophase, TFH) on experimental oxidative injuries of lens. METHODS: All fresh transparent lenses of rabbit eyes except control group were bathed in Fenton reaction system in order to produce a model of oxidative damages of lens, meanwhile Sch B, SIB, PG, SF, TFH and pirenoxine sodium (PS) were added in the reaction system in different groups respectively. Lenses were incubated for 24 hours. Then total protein (TP), soluble protein (SP), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione (GSH), vitamin C (Vit C), total activities of anti-oxidation (TAO) and malondiaoldehyde (MDA) in homogenized lenses were measured to observe the effects of five anti-oxidants on above index. RESULTS: Five anti-oxidants increased the anti-oxidative index and decreased MDA in lens of oxidative damages in different levels, the effects are better than that of PS, especially in group SF and Sch B. CONCLUSION: The five natural anti-oxidants protected lens against experimental oxidative injuries very well. There are wide prospects to pursue effective anti-cataract drugs from natural anti-oxidants.%目的：探讨五味子乙素（SchB）、水飞蓟宾（SIB）、没食子酸丙酯（PG）、阿魏酸钠（SF）和沙棘总黄酮（TFH）5种天然抗氧化剂对抗实验性晶状体氧化损伤的作用。方法：将兔眼透明晶状体温育在Fenton氧化反应模型中造成晶状体氧化损伤，同时分别加入上述5种天然抗氧化剂共同温育24h后，测定晶状体总蛋白和可溶性蛋白、SOD、GSH-Px、GSH、VitC、总抗氧化能力和MDA的水平，观察5种抗氧化剂对上述指标的影响。结果：5种抗氧化剂均不同程度地升高氧化损伤晶状体的抗氧化指标,降低脂质过氧化终末产物MDA含量,且优于白内停滴眼液。其中SF和SchB的作用更为显著。结论：5种天然抗氧化剂对实验性晶状体氧化损伤有良好的对抗作用，从天然抗氧化剂中寻找高效的抗白内障药物具有广阔的前景。
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