“一肾一夹”高血压大鼠肾脏皮质钠泵 α亚单位的基因表达

摘要

目的：探讨“一肾一夹(1k1c)”高血压大鼠肾脏皮质钠泵α亚单位各异构体基因表达的改变，为高血压发病机制的深入研究提供理论及实验依据。方法：分别应用RT-PCR及免疫组化技术，探讨1k1c高血压大鼠肾脏皮质钠泵α1、α2及α3亚单位mRNA及蛋白水平基因表达的改变。结果：正常大鼠肾脏皮质主要表达钠泵α1亚单位，α2与α3亚单位表达较弱；在mRNA水平，1k1c高血压大鼠肾脏皮质钠泵α1亚单位表达明显较强，α2与α3亚单位表达无改变，而在蛋白水平各亚单位基因表达与对照组比较均无明显差异。结论：1k1c高血压大鼠肾皮质钠泵α亚单位基因表达发生改变，这种改变可能参与该模型的血压升高机制。%AIM: To investigate the regulation of sodium pump α-subunit geneexpression in the cortex of kidneys from one kidney one clip (1k1c) hypertensive rats. METHODS: 1k1c hypertensive rats were prepared by partially ligating the left renal artery and removing the right kidney. 4 weeks later, all the rats were killed, the levels of sodium pump α1-, α2-, and α3-subunit mRNA and protein in the cortex of kidney were detected with reverse transcription polymerase chain reaction (RT-PCR) method and immunohistochemical assay, respectively. RESULTS: The mRNA levels of sodium pump α1-subunit increased and α2- and α3-subunit were unchanged in the cortex of kidneys from 1k1c hypertensive rats compared with control rats, while no change has been found for all the three subunits gene expressions at protein level. CONCLUSION: There were some changes in the expression of sodium pump α-subunit gene in the cortex of kidney of the 1k1c hypertensive rat, which might be related to the development of hypertension in this hypertensive model.