目的:观察肾组织载脂蛋白M(apoM)在缺血再灌注性急性肾衰(ARF)时的表达变化以及核因子-κB(NF-κB)特异性抑制剂-吡咯烷二硫代氨基甲酸盐(PDTC)对apoM表达的影响,探讨apoM是否参与ARF的发病机制.方法:建立大鼠缺血再灌注性急性肾衰模型,分为假手术组、ARF组和PDTC组.采用Western blotting,检测不同时点肾皮质apoM和核内NF-κB p65亚基的表达.结果:ARF组各时点apoM水平较假手术组明显增高(P＜0.01),且呈双峰型变化,于再灌注6 h达第1次高峰,之后表达逐渐下降,从24 h到48 h表达再次上调;PDTC组apoM的变化趋势与ARF组相同,但各时点apoM水平较ARF组显著降低(P＜0.01).ApoM与核内NF-κB p65亚基的表达呈明显正相关.结论:apoM可能通过NF-κB的介导参与缺血再灌注性ARF的发病.%AIM: To investigate the expression pattern of apolipoprotein M (apoM) protein in renal cortex of a-cute renal failure ( ARF) rats with reperfusion. METHODS: Seventy - five male rats were randomly divided into sham operation group (re =25) , ARF group (n =25) and pyrrolidine dithiocarbamat (PDTC) group (n =25) , five subgroups at time points of 3 h, 6 h, 12 h, 24 h and 48 h after reperfusion were set up in each group. The expressions of apoM in cytoplasm and NF - κB p65 in nucleus of renal cortex were detected at the indicated time points. RESULTS: The expression of apoM in ARF group was obviously higher than that in sham operation group ( P <0.01 ) , and two peaks were detected, the first peak was at 6 h after reperfusion, while the second one was from 24 h to 48 h. The tendency of apoM expression in PDTC group was similar to that in ARF group, while the expression in every subgroup was prevalently lower than that in ARF group (P < 0.01). Otherwise, a significant correlation ( r = 0.852, P < 0.01) was found between the expression of apoM and NF -κB p65.CONCLUSION: The results indicate that apoM feasibly take part in the pathogenesis of ARF through the inflammatory reaction mediated by NF - κB.
展开▼
