ATM; To investigate the inhibitory effect of survivin - siRNA recombinant plasmid on prostate cancer xenografts. METHODS; Prostatic cancer DU145 cells were cultured and subcutaneously injected into nude mice . When the tumor grew to 8 mm in diameter, it was aseptically removed and divided into about 2 mm blocks through surgery and subcutaneously implanted into another nude mice . After the prostatic cancer xenograft model was reconstructed , the mice were treated with survivin - siRNA plasmid and control scrambled siRNA plasmid using electric transfection method . The tumor growth curve was plotted and the inhibitory rate was calculated . HE staining, immunohistochemical staining and TUNEL assay were applied to observe the effect of survivin - siRNA on the xenografts. RESULTS; The prostatic cancer xenograft model was successfully constructed in vivo. Compared with mock and scrambled siRNA groups , transfection of survivin- siRNA recombinant plasmid obviously inhibited the tumor growth with the inhibitory rates of 61.81% and 62. 87% , respectively. Compared with both controls , survivin - siRNA depressed the protein expression of survivin and promoted the cell apoptosis. CONCLUSION; Survivin -siRNA recombinant plasmid significantly inhibits the growth of prostatic tumor xenografts by inhibiting the protein expression of endogenous survivin and promoting cell apoptosis .%目的:探讨survivin-siRNA真核重组质粒对前列腺癌移植瘤的抑瘤效应,并分析相关机制.方法:体外培养前列腺癌DU145细胞,将细胞注射到裸鼠背部皮下,待移植瘤直径达到8 mm时将瘤无菌取出,分成直径约2 mm的瘤块,手术植入另外的裸鼠皮下,将survivin-siRNA质粒或scrambled siRNA对照质粒电转染进行治疗,描记移植瘤生长曲线,并计算抑制率;通过HE染色、免疫组化染色及TUNEL染色观察survivin-siRNA对移植瘤影响.结果:成功构建裸鼠前列腺癌移植瘤模型.与mock和scrambled siRNA组相比,survivin-siRNA抑瘤率分别为两对照组的61 81%和62 87%;免疫组织化学染色发现:survivin-siRNA质粒明显抑制细胞内survivin表达;TUNEL染色证实治疗组肿瘤细胞凋亡明显增加.结论:重组质粒survivin-siRNA明显抑制DU145细胞移植瘤的生长,抑制内源性 survivin 蛋白的表达并促进细胞凋亡,针对survivin靶点的基因治疗前景广阔.
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