静脉输入休克大鼠肠淋巴液对正常大鼠红细胞的损伤作用

摘要

目的:观察静脉输入休克大鼠肠淋巴液对正常大鼠红细胞参数、代谢以及血液黏度的影响,探讨休克肠淋巴液对红细胞的损伤作用.方法:将引流休克1～3 h的大鼠肠淋巴液离心去细胞后,以等量生理盐水稀释,经股静脉输入正常大鼠(2 mL/kg),时间为30 min;另一组大鼠输入等量生理盐水作为对照组.输液结束后2.5 h,经腹主动脉取血,检测红细胞常规参数、三磷酸腺苷(ATP)、乳酸(LA)、2,3-二磷酸甘油酸(2,3-DPG)含量、内外液离子浓度以及血液黏度等指标.结果:静脉输入休克肠淋巴液降低了正常大鼠红细胞数目、血红蛋白浓度、血细胞比容和ATP含量,使红细胞平均体积、2,3-DPG和LA含量及全血还原黏度显著增高,但对红细胞内外液离子浓度、全血黏度和血浆黏度无明显影响.结论:静脉输入休克肠淋巴液引起正常大鼠红细胞能量代谢障碍,从而导致红细胞体积与全血还原黏度增加,即休克肠淋巴液可损伤红细胞.%AIM: To observe the effects of intravenous injection of the mesenteric lymph from shock rats on the characteristics and metabolism of red blood cells ( RBC ), and blood viscosity in normal rats. METHODS: The mesenteric lymph samples, collected from the rats 1 to 3 h after hemorrhagic shock, centrifuged to remove all cellular components and diluted with equal volume of saline, were intravenously injected into normal rats at dose of 2 mL/kg through femoral vein within 30 min. The equal volume of saline was intravenously injected into other normal rats as controls. At 2. 5 h after injection, the blood samples were collected from the abdominal aorta for determining the routine parameters, adenosine triphosphate ( ATP ), lactic acid ( LA ), 2, 3 — diphosphoglycerate (2,3— DPG ), ion concentrations of intra — and extracellular fluid of the RBC and blood viscosity. RESULTS: Intravenous injection of shocked mesenteric lymph reduced the number of RBC, the concentration of hemoglobin, the hematocrit and the content of ATP. Intravenous injection of shocked mesenteric lymph significantly increased the mean corpuscular volume ( MCV ), 2, 3 — DPG, LA in RBC and the whole blood reduced viscosity. However, no obvious effect of the injection on ion concentrations of intra - and extracellular fluid of RBC, whole blood viscosity and plasma viscosity was observed. CONCLUSION: Intravenous injection of shocked mesenteric lymph causes the disorders of energy metabolism in RBC, thus increasing the MCV and whole blood reduced viscosity. Shocked mesenteric lymph damages RBC.