参术冠心颗粒对心肌梗死大鼠冠脉微循环的影响

摘要

AIM: To explore the effect of traditional Chinese medicine Shenshuguanxin granula on coronary circulation in a rat model of myocardial infarction (MI).METHODS:SD rats (n=50, SPF grade) were randomly divid-ed into 5 groups (n =10): sham group, MI group, and high-dose, middle-dose and low-dose Shenshuguanxin granula treatment groups.The rat MI model was established by ligation of the coronary artery .The cardiac markers, small and me-dium-sized blood vessels [microvessel count (MVC) value] in the infarct zone, and platelet endothelial cell adhesion mo-lecule 1 (PECAM-1) and vascular endothelial growth factor (VEGF) expression in the infarct border zone were measured . RESULTS:After 4 weeks of coronary artery ligation , the significant increases in MVC in the infarct zone , and the expres-sion of PECAM-1 and VEGF in the infarct border zone were detected compared with sham group (P<0.05).The differ-ences of cardiac markers between MI group and other groups were insignificant (P>0.05).CONCLUSION: Shenshu-guanxin granula improves coronary circulation in the rats with myocardial infarction by increasing the expression of PECAM -1 and VEGF, and promoting small and medium-sized angiogenesis.%目的：探讨参术冠心颗粒（SSGX）对大鼠心肌梗死（MI）模型冠脉微循环作用。方法：健康SPF级SD大鼠50只，随机分成假手术（sham）组、MI组、SSGX高剂量、中剂量和低剂量治疗组，每组10只。后4组结扎冠状动脉建立MI大鼠模型，假手术组只穿线不进行结扎。造模3 d后超声检查淘汰不合格大鼠，药物干预4周，测定4周后各组大鼠血清心肌损伤标志物、梗死区中小血管平均血管面密度（MVC值）、梗死边缘区血小板内皮细胞黏附分子1（PECAM-1）和血管内皮生长因子（VEGF）表达。结果： MI组与sham组比较，光学显微镜见MI组死亡的心肌纤维间空隙变宽，密集的中型多形核白细胞浸润，肉芽组织增生，坏死心肌纤维被致密的胶原纤维取代。超声可见MI组左室室璧活动异常，射血分数减低。心肌钙蛋白T（cTnT）、MVC、PECAM-1和VEGF各指标与sham组比较均有显著差异（P＜0．01），证明造模成功。大鼠心肌梗死区中小血管的MVC、梗死边缘区PECAM-1及VEGF的表达，SSGX各剂量组与MI组相比表达明显增加，差异有统计学意义（P＜0．01），呈量效关系。但各SSGX组大鼠和MI组大鼠之间，心肌标志物差异无统计学意义（P＞0．05）。结论：参术冠心颗粒能改善心肌梗死大鼠冠脉微循环状态，其机制可能与增加PECAM-1和VEGF表达、促进中小血管新生有关。