冠脉显色指导下冠脉结扎法在制作大鼠心肌梗死模型中的应用

摘要

目的：显示大鼠左侧冠状动脉的走行情况，探讨冠脉显色指导下冠脉结扎法制作大鼠心肌梗死模型在模型死亡率、成功率及稳定性方面的优势。方法：8只SD大鼠处死后取出心脏，行主动脉插管后肝素盐水灌洗冠状动脉，1%、2%、3%、4%不同浓度37℃琼脂糖凝胶行主动脉根部灌注，确定最佳琼脂糖凝胶浓度；101只雄性SD大鼠行琼脂糖凝胶主动脉灌注，显示出大鼠冠状动脉的分布走行情况；分析冠脉走行情况，制定结扎策略，比较高位结扎法、低位结扎法在大鼠存活率、结扎成功率、梗死灶稳定性方面的优劣。结果：2%37℃琼脂糖凝胶主动脉灌注能清楚的显示冠状动脉在心脏表面的走行；大鼠冠脉的走行可见分为4种类型；60只雄性SD大鼠分别按高位结扎、低位结扎的方法能制作稳定的大鼠心梗模型。结论：冠脉显色指导下冠脉结扎法能制作出稳定的大面积和中等面积大鼠心肌梗死模型。%Objective:To show the anatomy of left coronary artery in rats and discuss the advantage of the death rate, success rate and stability of myocardial infarction model through ligating of the coronary artery under staining guidance.Method:8 heart harvested from sacrificed rat and irrigated coronary artery with saline injection after aortic intubation.The best concentration of agarose gel were identified, 1%, 2%, 3% and 4% agarose gel were used to perfuse aortic root at 37 ℃.101 male rats were perfused to show the anatomy of left coronary artery, then made ligation strategies though analyzed coronary artery distribution, and compared the advantages and disadvantages in death rate, success rate and advantage of stability between high and low ligation.Result:It showed the coronary artery clearly though aortic perfusion by 2% agarose gel at 37 ℃, and the coronary anatomy can mainly be classified into 4 groups. We had made stable myocardial infarction model both in high and low ligation.Conclusion:The method of ligation of the coronary artery under staining guidance can produce stable rat myocardial infarction model both in large and middle infarct size.