人参总皂苷促进再生障碍性贫血小鼠造血及其机制探讨

摘要

[ ABSTRACT] AIM:To observe the effects of total saponins of Panax ginseng ( TSPG) on the promotion of hema-topoiesis and to explore the underlying mechanism in treating aplastic anemia ( AA) in a mouse model.METHODS:For preparation of AA model, BALB/c mice were exposed to sublethal dose of 5.0 Gyγradiation, followed by transplantation of lymphocytes from DBA/2 donor mice.The experiment was divided into 6 groups, including normal control group, AA model group, TSPG treatment groups with low, medium and high doses, and positive control group with cyclosporine A ( CsA) .Both TSPG and CsA were administered by gastrogavage.After 15 d treatment, the peripheral hemogram was test-ed, the cytokine contents in serum were measured, and bone marrow semisolid culture of colony-forming assay was conduc-ted for determining hematopoietic progenitor cells.The protein levels of extracellular signal-regulated kinase 1/2 ( ERK1/2) and its phosphorylation status in the bone marrow cells were determined.RESULTS:Curative effect of TSPG in trea-ting AA mice was satisfactory.Peripheral counts of white blood cells and platelet, and concentration of hemoglobin in TSPG groups with medium and high doses were significantly higher than those in model control.TSPG increased the colony num-bers of CFU-E, BFU-E, CFU-GM and CFU-MK derived from hematopoietic progenitor cells.Meanwhile, up-regulation of the phosphorylated ERK1/2, decreased contents of Th1 cytokines and increased contents of Th2 cytokines in the serum were observed.CONCLUSION:TSPG possess the dual efficacies on the promotion of hematopoiesis and immunoregulation in AA mice by regulating the expression of Th1/Th2/Th17 cytokines and increasing the phosphorylation of ERK1/2.%目的：观察人参总皂苷（ TSPG）对免疫介导型再生障碍性贫血（再障）小鼠的疗效及其相关作用机制。方法：BALB／c小鼠经亚致死剂量照射后输入DBA／2小鼠淋巴细胞，制成再障小鼠模型。实验分6组，正常小鼠组，模型组，TSPG低、中和高剂量治疗组，环孢菌素A（ CsA）阳性对照组，灌胃给药15 d。观察外周血象，检测血清Th1／Th2／Th17因子含量、骨髓造血祖细胞集落生成率及细胞外信号调节激酶（ extracellular signal-regulated kinase 1／2，ERK1／2）的磷酸化水平。结果：TSPG治疗再障小鼠的疗效显著，中、高剂量组的血红蛋白含量、白细胞和血小板计数均明显高于模型组；红、粒和巨核系造血祖细胞集落生成率均明显高于模型组。同时，显著上调骨髓细胞磷酸化ERK1／2蛋白表达，降低血清Th1和Th17因子含量，并升高Th2因子含量。结论：人参总皂苷有效地治疗再障小鼠，具有一定程度的促进造血和调节免疫的功效，其作用机制可能是调节Th1／Th2／Th17因子的表达及ERK1／2的磷酸化。