扰乱昼夜节律对小鼠角膜创伤修复的影响

摘要

目的：本研究旨在观察昼夜节律对角膜创伤修复的影响。方法：选用C57 BL／6雄性小鼠，在小鼠角膜中央直径为2 mm的圆形区域，用高尔夫样刀机械性去除其上皮层，使用荧光素钠显色创伤面积，动态观察上皮层修复的动力学，并观察白细胞、血小板和分裂细胞的动态变化。结果：全昼（12 h light／12 h light， LL）和全夜（12 h dark／12 h dark， DD）组角膜创伤修复速率小于对照组（12 h light／12 h dark， LD），表现为再上皮化延迟、上皮细胞数量降低、血管扩张直径增大、白细胞和血小板募集的发生延迟，但白细胞和血小板数目显著增多。结论：扰乱昼夜节律延迟炎症反应的发生，但加剧炎症反应；延迟再上皮化过程，最终显著抑制角膜创伤修复过程。%[ ABSTRACT] AIM: To observe the effect of circadian rhythms on wound healing of mouse corneal epithelium. METHODS:The C57BL/6 male mice were used in the study.A part of corneal epithelium (2 mm in diameter) was struck off by a golf-like knife to form a round wound area.The dynamics of epithelial healing in the wound area were ob-served under microscope with fluorescein staining.In addition, with related antibodies and DAPI, the dynamic changes of the neutrophils, platelets and dividing cells were also investigated.RESULTS:The healing rates in LL group (12 h light/12 h light) and DD group (12 h dark/12 h dark) were obviously slower than that in LD group (12 h light/12 h dark), mainly showing delayed re-epithelialization, decreased epithelial cells, increased diameter of blood vessel, and delayed re-cruitment of neutrophils and platelets, but more cell number.CONCLUSION:Disruption of circadian rhythms significant-ly inhibits the wound healing of corneal epithelium, mainly through delaying the inflammation and re-epithelialization, but aggravating the inflammatory responses.