Fra-1与HMGA1基因在喉鳞状细胞癌中的表达及相关性研究

摘要

Objective To investigate the expressions of Fra-1 and HMGA1 in laryngeal squamous cell carcinoma and their correlation.Methods Immunohistochemistry and reverse transcription-polymer chain reaction (RT-PCR) were used to detect the expressions of HMGA1 and Fra-1 in laryngeal squamous carcinoma tissues in 47 cases and para-carcinolna tissues in 21 cases(the First Hospital of Shijiazhuang).The relationship between the gene expressions in carcinoma tissues and clinopathological parameters such as pathological grade,clinical stage,lymph metastasis,age and anatomic site and the relevance of the two gene expressions were analyzed.SPSS 13.0 software was used to analyze the data.Results The positive expression rates of Fra-1 and HMGA1 proteins in laryngeal squamous cancer tissue were 48.9％ and 53.2％,which were respectively higher than the rates of 19.0％ for Fra-1 (x2 =5.416,P ＜0.05) and of 23.8％ for HMGA1 (x2 =5.083,P ＜ 0.05) in adjacent tissues.The expression of Fra-1 gene was correlation with pathological grade,clinical stage and lymph metastasis (t values were-1.079,-1.066 and-1.067,all P ＜0.05),but not with age and anatomic site (t values were-1.068 and-1.054,both P ＞ 0.05).The expression of HMGA1 gene was correlation with pathological grade,clinical stage,lymph metastasis and age (t values were-1.112,-1.065,-1.009 and-1.066,all P ＜0.05),but not with anatomic site (t =-1.036,P ＞ 0.05).The expressions of Fra-1 and HMGA1 gene were positively correlation (r =0.672,P ＜ 0.05).Conclusions In laryngeal squamous cancer,Fra-1 and HMGA1 are excessive expression,with a positive correlation between the expressions of both genes.%目的 探讨喉鳞状细胞癌(squamous cell carcinoma,SCC)组织中Fra-1(Fos related antigen-1)、HMGA1(high mobility group protein A1)基因的表达及其间的相关性.方法 应用免疫组化SP法及反转录-聚和酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测石家庄市第一医院47例喉SCC组织和21例癌旁正常组织中Fra-1和HMGA1基因的表达情况,在癌组织中分组探讨两者与病理分级、临床分期、淋巴结转移、年龄、解剖分区的关系,并进一步分析两基因表达的相关性.应用SPSS 13.0统计软件进行统计学分析.结果 Fra-1 、HMGA1蛋白在喉SCC组织中阳性率(48.9％、53.2％)均高于癌旁正常黏膜组织(19.0％、23.8％),差异有统计学意义(x2值分别为5.416、5.083,P值均＜0.05).Fra-1基因表达与病理分级、临床分期、淋巴结转移相关(t值分别为-1.079、-1.066、-1.067,P值均＜0.05);与年龄、解剖分区无关(t值分别为-1.068、-1.054,P值均＞0.05).HMGA1基因表达与病理分级、临床分期、淋巴结转移、年龄相关(t值分别为-1.112、-1.065、-1.009、-1.066,P值均＜0.05);与解剖分区不相关(t=-1.036,P＞0.05).Fra-1基因与HMGA1基因表达呈正相关(r=0.672,P＜0.05).结论 喉SCC组织中Fra-1和HMGA1基因均过度表达,此两种基因呈正相关.