半胱氨酸链蛋白β在内耳中的表达及其与听功能的相关性研究

摘要

Objective To investigate the expression of cysteine string protein beta(CSPβ)in the inner ear in Bama miniature pigs and its dynamic changes during the development of C57 mice;and to study its correlation with hearing function.Methods The distribution of CSPβ was detected by immunofluorescence on frozen sections and on whole co-chlear basilar membrane preparations during different developmental stages of cochlear maturation.Auditory brainstem responses (ABRs) were recorded in developing C57 mice.One-way analysis of variance using the SPSS23.0 software was employed in results analysis.Results CSPβ was localized in the cytoplasm of spiral ganglion cells and inner hair cells rather than in outer hair cells in adult C57 mice.During cochlear maturation,there was no expression of CSPβ in inner hair cells in C57 mice until postnatal day 12.In addition,fluorescence signals increased during development while outer hair cells lacked CSPβ signals all the time.Unlike C57 mice,CSPβ expression was seen in inner hair cells in min-iature pigs within 1 day of birth.ABRs were first recorded by postnatal day 14,and a decrease in latency and threshold was observed in the weeks following hearing onset.Conclusion We believe that there may be a close link between CSPβ and neurotransmitter release from inner hair cells,probably a prerequisite for neurotransmitter release,informa-tion transmission and hearing onset.%目的 研究半胱氨酸链蛋白β(cysteine string proteinβ,CSPβ)在巴马小香猪及出生后不同日龄的C57小鼠内耳组织中的表达,探讨其与听功能的相关性.方法 应用耳蜗冰冻切片免疫荧光染色观察CSPβ在内耳中的表达分布,应用基底膜铺片观察CSPβ随生长发育的表达变化.应用ABR检测C57小鼠发育过程中的听力变化.结果 采用SPSS23.0统计学软件进行各组间单因素方差分析.结果 CSPβ在成年C57小鼠的螺旋神经节以及内毛细胞中均有表达,而在外毛细胞中没有表达;出生12天之前的C57小鼠耳蜗基底膜内毛细胞中都没有CSPβ的表达,直到出生后第12d,内毛细胞中才出现CSPβ的表达,且随着小鼠日龄的增加,CSPβ的表达信号增强,而在外毛细胞中始终没有出现CSPβ的表达.与C57小鼠不同的是,出生1天的小型猪内毛细胞胞中即有CSPβ表达,但在3排外毛细胞中没有表达;ABR结果显示自小鼠出生第14d开始检测到ABR,随着日龄的增加听力越来越成熟.结论 我们推测CSPβ与内毛细胞释放神经递质之间可能存在密切联系,可能是神经递质释放,信息传递以及听觉发生的前提条件之一.