金天格胶囊对膝骨关节炎患者关节液中 MMP－3、TIMP－1、IL－1β、TGF－β1水平的影响

摘要

目的：探讨金天格胶囊对膝骨关节炎（knee osteoarthritis，KOA）患者关节液中基质金属蛋白酶－3（MMP－3）、基质金属蛋白酶组织抑制剂－1（TIMP－1）、白细胞介素－1β（IL－1β）及转化生长因子－β1（TGF－β1）的影响。方法选取90例KOA患者，分别在治疗前、治疗1个疗程、治疗2个疗程后，对患者进行WOMAC量表评分，评估患者膝关节功能。分别抽取患者膝关节腔关节液，进行酶联免疫吸附法（ELISA），测定关节液中MMP－3、TIMP－1、IL－1β、TGF－β1的含量。结果（1）服用金天格胶囊后，患者WOMAC评分降低，膝关节功能改善。（2）与治疗前相比，治疗后关节液中MMP－3、IL－1β含量都有所降低，TIMP－1、TGF－β1含量都有所增加，差异具有统计学意义（P＜0．01）。（3）随着服用金天格胶囊疗程的增加，膝关节液中MMP－3、IL－1β含量逐渐下降，TIMP－1、TGF－β1的含量逐渐升高，组间差异具有统计学意义（P＜0．01）。结论金天格胶囊可以降低KOA患者关节液中MMP－3、IL－1β水平，升高TIMP－1、TGF－β1的水平，从而起到保护关节软骨、改善KOA患者膝关节功能的作用。%Objective To evaluate the effect of Jintiange capsule on the levels of MMP-3, TIMP-1, IL-1β, and TGF-β1 in synovial fluid of patients with knee osteoarthritis ( KOA) .Methods Ninety KOA patients were involved.WOMAC scores were used to evaluate the knee joint function before the treatment and after one and two courses of the treatment.The synovial fluid in knee joint was collected and the levels of MMP-3, TIMP-1, IL-1β, And TGF-β1 in the synovial fluid were detected using ELISA method.Results 1) After oral intake of Jintiange capsule, WOMAC scores of the patients decreased significantly, and the knee joint function improved.2) The levels of MMP-3 and IL-1βin synovial fluid after the treatment gradually decreased comparing to those before the treatment, and the levels of TIMP-1 and TGF-β1 in the synovial fluid gradually increased ( P <0.01 ) .3 ) Following the increase of the time course of Jintiange treatment, the levels of MMP-3 and IL-1βin synovial fluid decreased and the levels of TIMP-1 and TGF-β1 increased.The differences between the groups were significant ( P<0.01 ) .Conclusion Jintiange capsule can markedly decrease the levels of MMP-3 and IL-1β, and increase the levels of TIMP-1 and TGF-β1 in synovial fluid. Thus, Jintiange Capsule can protect the articular cartilage and improve the knee joint function of KOA patients.