大鼠大脑新皮质组织移植后免疫组织化学及辣根过氧化物酶追踪的研究

摘要

The fetal neocortical transplant(E15～17 days of gestation)of Wistar rats was grafted to the corresponding neocortical region of young rats of same strain.The immunohis tochemieal study and horseradish peroxidase(HRP)retrograde tracing were performed on the 7th,15th,30th,60th,150th day after transplantation.The results showed thai most grafted neurons survived,grew,differentiated and matured.The morphology of neurons was similar to the structure of the neocortical neurons of host brain;integration of varying degree at the interface and reciprocal connection between graft and host brain tissue were established.%采用孕15～17天的Wistar大鼠胎脑新皮质组织移植到同种幼鼠大脑新皮质的相应区.分别于术后7、15、30、60、150天应用免疫组织化学和辣根过氧化物酶(HRP)追踪观察移植物存活、生长分化及其与宿主脑的整合和神经纤维联系的相关性.结果表明,大部分移植物内的幼稚神经元不仅能存活,且能生长分化直至成熟,其形态结构和宿主脑神经元相似,移植物和宿主脑界面建立不同程度的整合,部分存活的移植物和宿主脑间形成纤维径路联系.