IL-1β和TNF-α在种植体和天然牙周围龈沟液中的比较研究

摘要

目的：本研究旨在探讨健康种植体和天然牙周围龈沟液的IL-1β和TNF-α的水平，为维持种植体周围组织的健康提供理论依据。方法：本研究共纳入17名患者，每名患者含有一颗健康种植牙和一颗同名天然牙(种植牙组：17枚ITI种植体；天然牙组：17颗天然牙)。采用酶联免疫吸附法对健康种植体和天然牙周围龈沟液的IL-1β和TNF-α进行分析；采用Spearman法对两组的IL-1β和TNF-α的水平相关性进行分析。结果：与天然牙组相比较，种植牙组的IL-1β和TNF-α的水平均增加。种植牙组和天然牙组的IL-1β水平分别为（Mean±SD，26．14±10．26；21．93±6．43），但两组间的IL-1β水平无统计学差异（P>0．05)；种植牙组和天然牙组的TNF-α水平分别为（Mean ±SD，3．17±0．72；2．67±0．58），两组间的TNF-α水平具有显著性统计学差异（P<0．05)。 IL-1β和TNF-α的水平相关性在种植牙组和天然牙组中均呈显著正相关（P<0．05)。结论：种植牙组的IL-1β和TNF-α的水平比天然牙组高。种植体周围结缔组织的成分和结构，菌斑以及种植体本身等因素可能使健康种植体周围龈沟液的IL-1β和TNF-α的水平比天然牙组高。%Objective: The purpose of this study was to describe levels of IL-1β and TNF-α in gingival crevicular fluid of healthy peri-implant and teeth, and to provide a theoretical basis for maintaining the healthy of the peri-implants tissues. Methods:17 patients were enrolled in this study. Each patient has a healthy dental implant and a tooth(implant group:17 ITI implants, tooth group:17 teeth). The levels of IL-1β and TNF-α in gingival crevicular fluid of the peri-implants and teeth were analyzed quantita-tively by using enzyme linked immunosorbent assay and the correlation between level of the IL-1β and TNF-α in two groups was analyzed by using the Spearman. Results: the levels of IL-1β and TNF-αwere higher around implants than teeth. The levels of IL-1β in dental implants and teeth were respec-tively (Mean ±SD, 26.14 ±10.26; 21.93 ±6.43), but the difference of the levels of IL-1β between two groups was not statistically significants (P>0.05). The levels of TNF-α of implants and teeth were re-spectively(Mean±SD, 3.17±0.72;2.67±0.58), and the difference of levels of TNF-α between two groups was statistically significant (P<0.05). Significant positive correlation was noted between the IL-1β and TNF-α in implants group(P<0.05), and in teeth group(P<0.05). Conclusion: The levels of IL-1β and TNF-α in gingival creviscular fluid of healthy implants were higher compared with the natural teeth. The composition and structure of connective tissue around the implants, plaque and the implant itself, may make more production of the IL-1β and TNF-α in gingival creviscular fluid around the healthy implants than teeth.