人突变Lumican基因转基因小鼠屈光变化特点

摘要

Objective To investigate the change of refractive status in transgenic mice with mutant Lumican (bright proteoglycan) gene at different ages. Methods Experimental Study. Fifty⁃four 3⁃week⁃old with mutant Lumican gene(cDNA 596T>C)mice (27 male and 27 female) were randomly divided into 9 groups (n=6,half male and half female) by random number table. One group (3⁃week⁃old) was randomly chosen and measured the refractive status by retinoscopy after mydriasis. Measurement of other groups were repeated the method above respectively in the fourth, fifth, sixth, eighth, tenth, twelfth, sixteenth, and twentieth week. Differences of diopter between right and left eye and between male and female were compared within each group by paired t test. The differences of mice's diopters in different age were compared by Kruskal-Wallis H test. Pairwise comparisons were acquired by Mann-Whitney U test. Results There were no statistic difference of diopters between binoculus:The mice's diopters of right and left eyes were respective measured in the twentieth week(1.50 ± 0.45)D and(1.25 ± 0.42)D (t=-0.889, P>0.05),The mice's diopters of right and left eyes were respective measured in the third week(-2.50±2.59)D and(-2.50 ± 4.32)D(t=0.000, P>0.05);There were no statistic difference of diopters between different genders:The mice's diopters of female and male were respective measured in the third week(-0.5±3.83)D and(-4.17±1.94)D,(t=2.079,P>0.05),The mice's diopters of female and male were respective measured in the twelfth week(1.50±0.84)D and(1.50±1.87)D (t=0.000, P>0.05);Analysis of binocular diopters revealed significant differences among nine groups(H=20.910,P<0.05).Diopters measured in the third week(-2.50 ± 3.40D) and the sixth week(+3.25 ± 2.67)D had statistical difference(Z=-3.259,P<0.001).There were no statistical significance between other groups (P>0.001). Conclusions Characteristics of diopters gathered from mice with Lumican gene mutation at different weeks are summarized as follows: Myopia could be observed in the third week. And this situation of myopia was gradually transformed into hyperopia with aging. The maximum hyperopic diopter was observed at 6th⁃week⁃old mice. From the eighth to twentieth week, the degree of hyperopic diopter gradually decreased and stabilized.%目的：探讨人突变Lumican基因转基因小鼠生后不同时间屈光状态及其变化特征。方法实验研究。54只（雄性27只，雌性27只）人突变Lumican基因（cDNA 596T>C）转基因小鼠，于生后3、4、5、6、8、10、12、16、20周共9个时间点分别通过随机数字表法随机选取6只（雄性3只，雌性3只）转基因小鼠，快速散大瞳孔后检影验光测量屈光度数。对比相同时间点转基因小鼠双眼间及雌雄性别间的屈光度数，采用配对t检验；不同时间点转基因小鼠屈光度数的比较采用非参数多个独立样本Kruskal⁃Wallis H检验，两两组间屈光度数比较采用非参数两个独立样本Mann⁃Whitney U检验。结果同周龄组转基因小鼠双眼间比较屈光度数差异无统计学意义：第20周转基因小鼠右左眼屈光度均值分别是（1.50±0.45）和（1.25±0.42）D，（t=-0.889，P>0.05），第3周转基因小鼠右左眼屈光度均值分别是（-2.50±2.59）和（-2.50±4.32）D，（t=0.000，P>0.05）；同周龄组不同性别转基因小鼠眼间比较屈光度数差异无统计学意义：第3周转基因小鼠雌雄性别眼屈光度均值分别是（-0.5±3.83）和（-4.17±1.94）D，（t=2.079，P>0.05），第12周转基因小鼠雌雄性别眼屈光度均值分别是（1.50±0.84）和（1.50±1.87）D（t=0.000，P>0.05）；不同周龄组转基因小鼠屈光度数差异有统计学意义（H=20.910，P<0.05）；两两组间比较显示转基因小鼠第3周与第6周屈光度数差异有统计学意义（Z=-3.259，P<0.001），其余各周之间比较屈光度数差异均无统计学意义（P>0.001）。结论人突变Lumican基因转基因小鼠出生后3周时为近视，随时间增加向远视发展，第6周远视屈光度数达到最大，8～20周时远视屈光度数逐渐减小并趋于稳定。（中华眼科杂志，2015，51：527-531）