微小RNA-218在胃癌中的表达与作用

摘要

Objective To investigate the expression and function of miR-218 in gastric cancer.Methods miR-218 levels were evaluated in 20 non-cardia gastric cancer tissues using TaqMan stem-loop real-time PCR analysis.Pre-miR-218 and anti-miR-218 inhibitor were used to change the miR-218 expression level and examine its effects on cell proliferation,apoptosis,cell cycle and cell invasion.Results Comparing with the corresponding normal tissues,miR-218 expression was significantly reduced in the gastric cancer tissue(P＜0.01).Forced expression of miR-218 increased apoptosis in AGS cells.The proportion of apoptosis cells induced by transfection of pre-miR-218 was greater than that induced by control(21.6% vs.10.4%,P=0.032).Pre-miR-218 resulted in a significantly decreased cell growth activity(P＜0.01)and cell invasion(P＜0.05)of AGS cells compared with that of the control.Conclusion miR-218 expression is reduced in gastric cancer.miR-218 may function as a tumor suppressor in gastric carcinoma.%目的 检测微小RNA-218(miR-218)在胃癌组织中的表达水平及其功能,探讨miR-218在胃癌发生、发展中的作用和意义.方法 采用发夹状引物的逆转录聚合酶链反应(RT-PCR)技术,检测20例非贲门胃癌组织和正常胃黏膜组织中miR-218的表达水平.通过转染不同的外源性分子,改变胃上皮AGS细胞miR-218的表达水平,检测miR-218对细胞增殖、凋亡、细胞周期和侵袭能力的影响.结果 与正常胃黏膜组织相比,miR-218在胃癌组织中呈低表达(P＜0.01),其比值平均为0.19(范围0.01～0.70).流式细胞技术检测结果显示,转染miR-218前体的AGS细胞凋亡率(21.6%)明显增加,与转染阴性参照(10.4%)的AGS细胞差异有统计学意义(P＜0.05).与转染阴性参照比较,转染miR-218前体可明显抑制细胞增殖(P＜0.01).Transwell试验证实,AGS细胞在转染miR-218前体后,细胞侵袭能力明显减弱(P＜0.05).结论 在胃癌组织中,miR-218的表达降低,miR-218具有促进细胞凋亡、抑制细胞增殖和侵袭的功能.